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Protocols

Delta2D specifications

Information


Unique identifier OMICS_02537
Name Delta2D
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

Versioning


No version available

Delta2D citations

 (21)
call_split

Identification of T helper (Th)1 and Th2 associated antigens of Cryptococcus neoformans in a murine model of pulmonary infection

2018
Sci Rep
PMCID: 5805727
PMID: 29422616
DOI: 10.1038/s41598-018-21039-z
call_split See protocol

[…] Spots of interest were mapped by overlaying Ponceau-stained nitrocellulose membranes, immunoblots and Coomassie-stained gels. Mapping was carried out with the software Delta2D (DECODON, Greifswald, Germany). The spots were excised manually from Coomassie-stained gels and digested in situ with trypsin. As described previously, the resulting peptides were eluted out o […]

call_split

Small RNA mediated repression of subtilisin production in Bacillus licheniformis

2017
Sci Rep
PMCID: 5516005
PMID: 28720814
DOI: 10.1038/s41598-017-05628-y
call_split See protocol

[…] protein separation in the second dimension. Gels were stained with Lava Purple (Fluorotechnics, Sydney, Australia) according to the instructions of the manufacturer. Gel images were analyzed with the Delta2D software (Decodon, Greifswald, Germany). Spot quantification was done according to Wolf et al.. Briefly, gel images (three replicates) from the wild type samples were overlaid with the gels fr […]

call_split

Bacillus pumilus KatX2 confers enhanced hydrogen peroxide resistance to a Bacillus subtilis PkatA::katX2 mutant strain

2017
Microb Cell Fact
PMCID: 5406934
PMID: 28446175
DOI: 10.1186/s12934-017-0684-y
call_split See protocol

[…] o the manufacturer`s instructions. 2D-PAGE was done with three independent biological replicates.Analysis of the gel images and spot quantification was performed as described by Wolf et al. using the Delta2D software version 4.4 (Decodon) []. Protein spots were excised from the gels (Ettan Spot Picker, GE Healthcare), digested and spotted onto MALDI targets (Ettan Spot Handling Workstation, GE Hea […]

library_books

Immunoproteomic identification and characterization of Ni2+ regulated proteins implicates Ni2+ in the induction of monocyte cell death

2017
PMCID: 5386519
PMID: 28300831
DOI: 10.1038/cddis.2017.112

[…] Fujifilm) and scanned gels were cooled (4 °C) until spot picking process and protein species identification was executed., Statistical analyses of stained or labeled samples were performed using the Delta2D software (Decodon, Greifswald, Germany). […]

library_books

Adaptive immune response to lipoproteins of Staphylococcus aureus in healthy subjects

2016
Proteomics
PMCID: 5096053
PMID: 27324828
DOI: 10.1002/pmic.201600151

[…] Protein spots of interest were identified by matching the superimposed 2D immunoblot images with the corresponding Flamingo‐stained 2D gel images by the Delta2D software v4.4 as described elsewhere . Protein identification by MS was carried out on a Proteome‐Analyser 4800 (AB Sciex, Framingham, USA). GPS explorer v3.6 (AB Sciex) and the MASCOT search […]

library_books

Contribution of the Twin Arginine Translocation system to the exoproteome of Pseudomonas aeruginosa

2016
Sci Rep
PMCID: 4899797
PMID: 27279369
DOI: 10.1038/srep27675

[…] t mutant exoproteomes were aligned using a warp transformation. Before spot detection and quantification was performed, a fused 2D gel of both images was created using the ‘union fusion’ algorithm of Delta2D. Spot detection was performed in the fusion gel containing all spots present in both images according to the automatically suggested parameters for background subtraction, average spot size, a […]

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Delta2D institution(s)
DECODON GmbH, Greifswald, Germany

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