Delta2D statistics

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Delta2D specifications

Information


Unique identifier OMICS_02537
Name Delta2D
Software type Package/Module
Interface Graphical user interface
Restrictions to use License purchase required
Operating system Windows
License Commercial
Computer skills Medium
Stability Stable
Maintained Yes

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Delta2D in publications

 (104)
PMCID: 5855798
PMID: 29443940
DOI: 10.3390/ijms19020576

[…] ma/30 w in 0.025 m tris/glycine ph 8.3 buffer. after electrophoretic separation, proteins were silver-stained, scanned using the image scanner iii (ge healthcare, warsaw, poland), and processed by delta2d software (version 4.7, decodon, greifswald, germany) []. statistical analyses were performed to select spots for further proteomic analyses, after manually excluding false-positive […]

PMCID: 5805727
PMID: 29422616
DOI: 10.1038/s41598-018-21039-z

[…] 1:500 were also tested., spots of interest were mapped by overlaying ponceau-stained nitrocellulose membranes, immunoblots and coomassie-stained gels. mapping was carried out with the software delta2d (decodon, greifswald, germany). the spots were excised manually from coomassie-stained gels and digested in situ with trypsin. as described previously, the resulting peptides were eluted […]

PMCID: 5799319
PMID: 29402985
DOI: 10.1038/s41598-018-20919-8

[…] gels indicated no major changes in the overall number of protein spots and their abundance in oass overexpressing and control nodules. when stained gels were analyzed for proteome differences using delta2d v3.6 image analysis software, a few unique protein spots were found in oass overexpressing nodules (fig.¬†). three protein spots with apparent molecular weight of 34‚ÄČkda, but with different […]

PMCID: 5787487
PMID: 29416634
DOI: 10.18632/oncotarget.22640

[…] 200 pmol of either scidye3- or scidye5 and the internal protein standard was labeled with 200 pmol of the scidye2. 2de separation was performed as recently described []. for gel image analyses the delta2d software package (decodon gmbh, greifswald, germany) was used. whereas differentially expressed proteins were classified by a fold change ratio of 1.5 (increased or decreased) and a p-value […]

PMCID: 5722575
PMID: 29228623
DOI: 10.18632/oncotarget.22135

[…] biological replicates with four to six technical replicates (gels) were used for proteome analysis. coomassie stained gels were documented with a gel scanner and gel pictures were compared with the delta2d software (decodon). the cut-off for up and down-regulation of protein spot was < 0.5 and > 2.5. putative creb-regulated proteins were cut-out with an automatic spot picker (spothunter, […]


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