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Deqor specifications

Information


Unique identifier OMICS_04733
Name Deqor
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability Stable
Maintained Yes

Publication for Deqor

Deqor in publications

 (21)
PMCID: 4602042
PMID: 26459602
DOI: 10.1083/jcb.201311117

[…] using endoribonuclease-prepared sirna (esirna), produced and purified as described elsewhere (). in brief, optimal esirna 400–600 bp target regions were selected within a gene of interest using the deqor web server (). the selected region was first amplified from hela cdna with specific primers that contained a t7 promoter sequence (underlined): appl1 forward, […]

PMCID: 4525174
PMID: 26215380
DOI: 10.1038/ncomms8822

[…] the genes to be knocked down were selected randomly, except that their annotations were based on rna sequence data, which may have led to some enrichment of highly expressed genes (see ). using the deqor prediction algorithm, templates were selected for high rnai efficiency and a low number of possible off-target sites., dsrna fragments with an average length of 479 bp were injected […]

PMCID: 4485866
PMID: 26121980
DOI: 10.1186/s12864-015-1614-1

[…] pools of esirna are verified by dna sequensing and gel electrophoresis to ensure identity and high specificity (sigma aldrich). to ensure high specificity and efficacy of the esirna, the algorithm deqor is utilized (design and quality control of rnai, available to the public via http://deqor.mpi-cbg.de/deqor_new/input.html). cflar specific 424 base pair sequence used in this study to create […]

PMCID: 4474930
PMID: 26091260
DOI: 10.1371/journal.pone.0130008

[…] 3’ and gsk3β-t7r: 5′-taatacgactcactataggggctcttgctctgtgaagttgaa- 3’. the dsrna specificity was estimated from similar genes in other species in the dscheck and deqor softwares [–]., three aliquots of eggs (150 mg each) were collected in microtubes and submitted to de-waxed treatment before electroporation was composed of three steps. in the first step, 400 […]

PMCID: 4336647
PMID: 25381480
DOI: 10.1007/s12026-014-8562-8

[…] the sirnas targeting the murine socs1 gene were designed using online sirna programs: sirna selection program (http://sirna.wi.mit.edu/home.php), sidirect version 2.0 (http://sidirect2.rnai.jp) and deqor v3 (http://deqor.mpi-cbg.de/deqor_new/input.html). the sirnas molecules used for suppression of murine socs1 gene and the negative control sirna (which does not target any sequence present […]


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Deqor institution(s)
Scionics Computer Innovation, GmbH, Dresden, Germany

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