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DESKGEN specifications


Unique identifier OMICS_08498
Software type Framework/Library, Package/Module
Interface Graphical user interface
Restrictions to use None
Input data Gene name
Operating system Unix/Linux
License Other
Computer skills Medium
Stability Stable
Maintained Yes


  • DESKGEN Cloud
  • DESKGEN CRISPR Libraries


No version available



  • person_outline DESKGEN Team

DESKGEN citations


Optimized RNP transfection for highly efficient CRISPR/Cas9 mediated gene knockout in primary T cells

PMCID: 5839763
PMID: 29436394
DOI: 10.1084/jem.20171626

[…] Routinely, three crRNAs were selected per target using the DESKGEN ( online platform. The target area was limited to the first ∼40% of the coding sequence, and preference was given to guides targeting different regions within this area. Guides […]


Trithorax dependent changes in chromatin landscape at enhancer and promoter regions drive female puberty

Nat Commun
PMCID: 5754362
PMID: 29302059
DOI: 10.1038/s41467-017-02512-1

[…] A panel of five sgRNAs targeting the putative Kiss1 enhancer site 1 was designed and cloned. The sgRNAs were designed using an online tool ( searching for the PAMs NNGRRT and NNGRR. Following phosphorylation and annealing of each oligonucleotide set, the double-stranded products were digested with BsaI (Eco31I), and ligated i […]


An efficient and scalable pipeline for epitope tagging in mammalian stem cells using Cas9 ribonucleoprotein

PMCID: 5947990
PMID: 29638216
DOI: 10.7554/eLife.35069.034

[…] annotated using SnapGene and the ~200 bp around the stop codon were used as an input for guide RNA designing. We designed guide RNAs using either the web-based tool form Desktop Genetics ( or our own bioinformatics ‘Tag-IN’ tool (below). High scoring guide RNAs were picked for synthesis (i.e those with cut sites in the 3’UTR, preferably within 8–15 bp distance from […]


R spondins can potentiate WNT signaling without LGRs

PMCID: 5800842
PMID: 29405118
DOI: 10.7554/eLife.33126.017

[…] Oligonucleotides encoding single guide RNAs (sgRNAs) () were selected from one of two published libraries (; ) or designed using the ‘Guide Picker’ tool of the DESKGEN Cloud CRISPR design software ( Oligonucleotides were cloned into the single cloning site of pX330 according to a published protocol () (original ver […]


Parallel genome wide screens identify synthetic viable interactions between the BLM helicase complex and Fanconi anemia

Nat Commun
PMCID: 5663702
PMID: 29089570
DOI: 10.1038/s41467-017-01439-x

[…] ng: For the generation of NQO1-CRISPR knock-out cells, the Cas9 double-nickase system was used. By combining the CRISPR Design Tool ( and the Desktop Genetics tool (, we selected a pair of two guide RNA (gRNAs) sequences of 20 base pairs each, targeting exon 3 of the human NQO1 gene (ENSG00000181019) with an offset distance of 9 base pairs. Th […]


Systematic discovery of mutation specific synthetic lethals by mining pan cancer human primary tumor data

Nat Commun
PMCID: 5460027
PMID: 28561042
DOI: 10.1038/ncomms15580

[…] A total of 20 bp oligonucleotide sequences targeting exon 4 of the ACACA locus were designed using the Desktop Genetics platform ( and four target sequences were chosen based on Rule Set Number 2. Annealed synthetic DNA oligos for each target sequence were phosphorylated and cloned into pX330-U6-Chimeric_BB-CBh-hSpCa […]


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DESKGEN institution(s)
Desktop Genetics, London, UK

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