DESKGEN specifications

Information


Unique identifier OMICS_08498
Name DESKGEN
Software type Framework/Library, Package/Module
Interface Graphical user interface
Restrictions to use None
Input data Gene name
Operating system Unix/Linux
License Other
Computer skills Medium
Stability Stable
Maintained Yes

Subtools


  • DESKGEN Cloud
  • DESKGEN CRISPR Libraries

Versioning


Add your version

Documentation


Maintainer


  • person_outline DESKGEN Team <>

DESKGEN in publications

 (9)
PMCID: 5839763
PMID: 29436394
DOI: 10.1084/jem.20171626

[…] was purified using the geneart precision grna synthesis kit according to the manufacturer’s protocol and eluted in 40 µl rnase-free water., routinely, three crrnas were selected per target using the deskgen (www.deskgen.com) online platform. the target area was limited to the first ∼40% of the coding sequence, and preference was given to guides targeting different regions within this area. […]

PMCID: 5754362
PMID: 29302059
DOI: 10.1038/s41467-017-02512-1

[…] england biolabs) for plasmid re-ligation., a panel of five sgrnas targeting the putative kiss1 enhancer site 1 was designed and cloned. the sgrnas were designed using an online tool (https://www.deskgen.com/) searching for the pams nngrrt and nngrr. following phosphorylation and annealing of each oligonucleotide set, the double-stranded products were digested with bsai (eco31i), and ligated […]

PMCID: 5800842
PMID: 29405118
DOI: 10.7554/eLife.33126.017

[…] experiments repeated at least twice., oligonucleotides encoding single guide rnas (sgrnas) () were selected from one of two published libraries (; ) or designed using the ‘guide picker’ tool of the deskgen cloud crispr design software (https://www.deskgen.com/landing/cloud.html). oligonucleotides were cloned into the single cloning site of px330 according to a published protocol () (original […]

PMCID: 5947990
PMID: 29638216
DOI: 10.7554/eLife.35069.034

[…] using snapgene and the ~200 bp around the stop codon were used as an input for guide rna designing. we designed guide rnas using either the web-based tool form desktop genetics (https://www.deskgen.com/landing/) or our own bioinformatics ‘tag-in’ tool (below). high scoring guide rnas were picked for synthesis (i.e those with cut sites in the 3’utr, preferably within 8–15 bp distance […]

PMCID: 5663702
PMID: 29089570
DOI: 10.1038/s41467-017-01439-x

[…] for the generation of nqo1-crispr knock-out cells, the cas9 double-nickase system was used. by combining the crispr design tool (http://crispr.mit.edu/) and the desktop genetics tool (https://www.deskgen.com/landing), we selected a pair of two guide rna (grnas) sequences of 20 base pairs each, targeting exon 3 of the human nqo1 gene (ensg00000181019) with an offset distance of 9 base pairs. […]


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DESKGEN institution(s)
Desktop Genetics, London, UK

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