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DeTCT specifications

Information


Unique identifier OMICS_09576
Name DeTCT
Alternative name Differential expression Transcript Counting Technique
Software type Package/Module
Interface Command line interface
Restrictions to use None
Operating system Unix/Linux
Computer skills Advanced
Stability Stable
Maintained Yes

Versioning


No version available

Documentation


Maintainer


  • person_outline Elisabeth M. Busch-Nentwich

Publication for Differential expression Transcript Counting Technique

DeTCT citations

 (3)
library_books

A high resolution mRNA expression time course of embryonic development in zebrafish

2017
eLife
PMCID: 5690287
PMID: 29144233
DOI: 10.7554/eLife.30860.051

[…] ()., using poly(a) pulldown rna-seq we have generated a comprehensive polyadenylated rna expression profile of normal zebrafish development. we have also used a 3′ end enrichment method called detct, developed by us for cost-effective high-throughput polya transcript screening (), to catalogue 3’ end usage. we have taken advantage of the large numbers of offspring and have divided the 5 […]

library_books

Proteolytic activity assayed by subcellular localization switching of a substrate

2016
PMCID: 5613695
PMID: 28955937
DOI: 10.1016/j.bbrep.2016.07.011

[…] previously . briefly, cells were fixed in 4% formaldehyde on ice for 5 min and then treated with methanol at 20 °c overnight. monoclonal antibody (mab) 9e10 against the myc-epitope was used to detct myc-tagged vp24 . the secondary antibody used for immunofluorescence was fluorescein isothiocyanate (fitc) labelled anti-mouse igg1. the cells were examined with confocal laser scanning […]

library_books

The Lymphatic Endothelial mCLCA1 Antibody Induces Proliferation and Growth of Lymph Node Lymphatic Sinuses

2016
PLoS One
PMCID: 4880189
PMID: 27224029
DOI: 10.1371/journal.pone.0156079

[…] popliteal ln sections were stained for lyve-1 and cd11b expression. cd11b+ macrophages coated the lyve-1+ lymphatic sinuses in both hamster igg- and 10.1.1 ab-injected mice. we were unable to detct any increase in macrophage representation or distribution on the growing sinuses in lns from 10.1.1 ab-treated mice (). moreover, the staining of cd11b+ macrophages (, arrows) and lyve-1+ lecs […]


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DeTCT institution(s)
Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, UK
DeTCT funding source(s)
The Wellcome Trust Sanger Institute (grant number WT098051), the EU Sixth Framework Programme (ZF-MODELS, contact number LSHG-CT-2003-503496); the EU Seventh Framework Programme (ZF-HEALTH)

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