The epigenetic regulator ATF7 controls adipocyte differentiation and thermogenic gene program (Affymetrix)

The adipocytes functions as a central organ in the regulation of metabolic homeostasis. Factors which contribute to the adipocyte differentiation and function would be the promising targets to combat the obesity and associated metabolic disorders. The activating transcription factor 7 (ATF7), a stress-responsive chromatin regulator, has recently been shown to be involved in the energy metabolism; however, the underlying mechanisms are still unknown. Here, we show that ATF7 is required for adipocyte differentiation and it interacts with histone dimethyltransferase G9a in adipocyte to repress the interferon-stimulated genes (ISGs) expression, which suppresses adipogenesis. Ablation of ATF7 promotes the beige biogenesis and browning of inguinal white adipose tissue (iWAT). ATF7 binds to the transcription regulatory regions of Ucp1 gene, and silences it by maintaining the histone H3K9 dimethylation level. These results establish the multifunction of ATF7 in adipocyte and provide molecular insights into the epigenetic control of development and function of adipose tissues. We used microarrays to identify the differentatial expression genes in the inguinal white adipose tissue of ATF7 KO mice.

Dataset features

Information

Specifications

Application:	Gene expression microarray analysis
Number of samples:	6
Release date:	Nov 10 2018
Last update date:	Nov 11 2018
Access:	Public
Diseases:	Brain Diseases, Metabolic
Genes:	ATF7, UCP1, Atf7
Taxon:	Mus musculus
Dataset link	The epigenetic regulator ATF7 controls adipocyte differentiation and thermogenic gene program (Affymetrix)

Experimental Protocol

Three control replicates (wildtype) and three experimental replicates (ATF7 KO) mice were analyzed.

Repositories

GEO

GSE122374

BioProject

PRJNA504815

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Contact

Yang LIU