Computational protocol: Secreted fungal sulfhydryl oxidases: sequence analysis and characterisation of a representative flavin-dependent enzyme from Aspergillus oryzae

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Protocol publication

[…] An in-house database containing 28 public fungal genomes [] was analysed by InterProScan program [] for the presence of a pyridine nucleotide disulphide oxidoreductases domain of class II and, in particular, FAD-dependent ones (IPR000103 and IPR013027 respectively). Presence of a target peptide for the secretory pathway was considered and determined with TargetP []. Multiple sequence alignments were produced with the software ClustalW [] and pairwise alignments at EMBL-EBI (http://www.ebi.ac.uk/Tools/emboss/align/index.html). […]

Pipeline specifications

Software tools InterProScan, TargetP, Clustal W, EMBOSS
Application Protein sequence analysis
Organisms Aspergillus oryzae, Triticum aestivum, Trichoderma reesei
Chemicals Glutathione, Hydrogen Peroxide, Oxygen