Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 64
Release date: Feb 1 2017
Last update date: Oct 24 2018
Access: Public
Chemicals: Methamphetamine
Dataset link RNA sequencing of C57BL/6J mice, heterozygous for knockout of Hnrnph1 gene, as well as C57BL/6J mice controls

Experimental Protocol


For this study, 3mm punches from both striatal hemispheres were collected and pooled from 16 mice (8 C57BL/6J controls (B6) and 8 C57BL/6J Hnrnph1 heterozygous knockouts (H), and RNA was extracted and prepared for cDNA library preparation using the Illumina TruSeq Stranded mRNA LT (100bp paired-end reads). Samples 1-16, consisting of alternating experiments were run in four lanes total (each sample run on each lane) across 3 different flow cells. Raw samples provided are labeled with the "H1-" prefix, followed by the the sample number and the lane that it was run (L001, L002, or L008) and paired-end read number (R1 or R2). The tail of the file name pertains to the flow cell number (0009, 0012, or 0014).

Repositories


GEO

GSE76929

ENA

SRP068520

BioProject

PRJNA308954

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Contact


Stefano Monti