|Application:||Gene expression microarray analysis|
|Number of samples:||4|
|Release date:||Apr 15 2008|
|Last update date:||Mar 19 2012|
|Chemicals:||Carbohydrates, Glucose, Hydrogen|
|Dataset link||Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus|
C. saccharolyticus was subcultured (overnight) 3 times on the substrate of interest in modified DSMZ 640 medium before inoculating a pH-controlled (pH = 7) 1-liter fermentor containing 4 gram substrate per liter. Cells were grown at 70 °C until mid-logarithmic phase (~OD660 = 0.3-0.4) and harvested by centrifugation and rapid cooling to 4 °C and stored at -80 °C. To elucidate the central carbon metabolic pathways and their regulation, transcriptome analysis was performed after growth on glucose, xylose and a 1:1 mixture of both substrates. L-Rhamnose, which was likely to follow another pathway, was used as a reference substrate.