Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Apr 15 2008
Last update date: Mar 19 2012
Access: Public
Chemicals: Carbohydrates, Glucose, Hydrogen
Dataset link Hydrogenomics of the extremely thermophilic bacterium Caldicellulosiruptor saccharolyticus

Experimental Protocol


C. saccharolyticus was subcultured (overnight) 3 times on the substrate of interest in modified DSMZ 640 medium before inoculating a pH-controlled (pH = 7) 1-liter fermentor containing 4 gram substrate per liter. Cells were grown at 70 °C until mid-logarithmic phase (~OD660 = 0.3-0.4) and harvested by centrifugation and rapid cooling to 4 °C and stored at -80 °C. To elucidate the central carbon metabolic pathways and their regulation, transcriptome analysis was performed after growth on glucose, xylose and a 1:1 mixture of both substrates. L-Rhamnose, which was likely to follow another pathway, was used as a reference substrate.

Repositories


GEO

GSE11153

ArrayExpress

E-GEOD-11153

BioProject

PRJNA106957

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