Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 12
Release date: Mar 31 2009
Last update date: Aug 28 2018
Access: Public
Dataset link ABA and ethylene signaling crosstalk

Experimental Protocol


Cold pre-treated seeds of wild type (Col), aba2 (or gin1-3), etr1-1, and ein2-1, were grown on 1% sucrose agar plates for 12 to 14 days. Total RNAs were extracted from which 10 μg aliquots were used for cDNA synthesis, labeling by in vitro transcription followed by fragmentation according to the GeneChip Expression Analysis Technical Manual rev5, Affymetrix. Eleven μg of each labeled samples was hybridized to an ATH1 GeneChip at 45oC for 16.5 hours. The washing and staining steps were performed using a Fluidic Station-450 and the ATH1 slides were scanned using the Affymetrix GeneChip Scanner 7G. Subsequent data processing and analysis was performed using the Affymetrix Microarray Suit Software version 5.0. Two independent sets of microarray analyses were performed in this study. The wild type in this study was used as a control.

Repositories


GEO

GSE12715

ArrayExpress

E-GEOD-12715

BioProject

PRJNA112651

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Contact


Wan-Hsing Cheng

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