Computational protocol: Resistance of Garlic Cultivars to Bradysia odoriphaga and Its Correlation with Garlic Thiosulfinates

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Protocol publication

[…] Raw data of all individuals in the life table were analyzed according to the age-stage two-sex life table theory. The developmental time, longevity, fecundity and population parameters and their mean values and standard errors were estimated using the bootstrap method included in the computer program TWOSEX-MS Chart, . Differences in B. odoriphaga populations among the cultivars were compared using the paired test included in the software.We tested the other variables for homogeneity of group variances using Levene’s test and normality using the Kolmogorov-Smirnov test prior to statistical analysis. For the analysis, the quantity of B. odoriphaga on each garlic cultivar was regarded as the dependent variable, while the garlic cultivars were regarded as the independent variables in one-way ANOVA followed by Tukey’s HSD multiple comparisons. With regards to the thiosulfinates content analysis at the same growth stages, the content in each garlic cultivar was regarded as the dependent variable, while the garlic cultivars were regarded as independent variables using one-way ANOVA followed by Tukey’s HSD multiple comparisons. Conversely, when analyzing the thiosulfinates content in the same garlic cultivar, the content at each growth stage was regarded as the dependent variable, while the growth stages were regarded as independent variables in the above-mentioned method. In the correlation analysis, thiosulfinates content in each cultivar and each growth stage of garlic was regarded as the independent variable, and B. odoriphaga larval development time, mortality, and fecundity reared on each cultivar were regarded as the dependent variables in the regression analysis followed by linear regression. In the bioassays, the number of dead B. odoriphaga larvae when treated with each insecticide dose (including the 0 mg kg−1 group, i.e., the control group) was regarded as the response frequency, while the doses were regarded as the covariate, and the total tested number of larvae in each treatment was regarded as the total observed in the regression analysis, which was followed by Probit. All analyses were performed with PASW Statistics 18.0.0 (2009; SPSS Inc. Quarry Bay, HK). Figures were constructed using SigmaPlot 12.0. […]

Pipeline specifications

Software tools SPSS, SigmaPlot
Application Miscellaneous
Diseases Drug-Related Side Effects and Adverse Reactions
Chemicals Sulfides