Computational protocol: Recombinant Haemonchus contortus 24 kDa excretory/secretory protein (rHcES-24) modulate the immune functions of goat PBMCs in vitro

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Protocol publication

[…] Sequence similarity was assessed using protein-protein basic local alignment search tools BLASTp and BLASTX sequences (http://www.blast.ncbi.nlm.nih.gov/Blast.cgi). HcES-24 sequences were aligned using ClustalX 1.83 program (http://www.clustal.org/). The phylogenetic tree was constructed by aligning the amino acid sequences using the Neighbor-Joining method and plotted and visualized using the Molecular Evolutionary Genetics Analysis 5.1 program (http://www.megasoftware.net/). […]

Pipeline specifications

Software tools BLASTP, BLASTX, Clustal W, MEGA
Application Phylogenetics
Organisms Haemonchus contortus, Capra hircus, Escherichia coli
Chemicals Nitric Oxide