Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 6
Release date: Mar 8 2017
Last update date: Sep 27 2017
Access: Public
Diseases: Angiolymphoid Hyperplasia with Eosinophilia, Graft vs Host Disease, Leukemia, Lymphoid, Neoplasms, Primary Myelofibrosis
Dataset link Gene expression profile (GEP) of miR-34a-5p-overexpressing CD34+ HPCs

Experimental Protocol


Human CD34+ cells were transfected by using the 4D-Nucleofector™ System (Lonza). Briefly, starting from the day after CD34+ cells purification, each sample was electroporated twice, once every 24 hours, with 3 µg of mirVana™ miR-34a-5p mimic or mirVana™ miRNA mimic Negative Control (NegCTR mimic). For each electroporation, 4×105 CD34+ cells were resuspended in 100 µL of P3 Primary Cell Solution (Lonza) containing 3 µg of miR-34a-5p mimic or NegCTR mimic and pulsed with the program DS112. Gene expression profile (GEP) was performed on total RNA derived from three independent experiments at 24h after the last nucleofection.

Repositories


GEO

GSE89156

BioProject

PRJNA350499

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Contact


Rossella Manfredini

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