Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 8
Release date: Mar 16 2012
Last update date: May 20 2013
Access: Public
Diseases: Protein Deficiency
Dataset link Transcriptomic response of skeletal muscle to lipopolysaccharide in the gilthead seabream (Sparus aurata)

Experimental Protocol


Total RNA from pooled control (n = 5) and LPS-treated (n = 5) sea bream white and red muscle tissues was labeled with Cy3-dUTP and Cy5-dUTP (GE Healthcare, Barcelona, Spain). We used a dye swap experimental design and each cDNA from a pooled RNA sample was hybridized to two microarrays. For the first slide, test and control cDNA were labeled with Cy5 and Cy3 respectively, and for the second array dye assignment was reversed. Therefore, samples from individual fish within each group were pooled and expression values shown represent the means of 6 × 2 = 12 technical replicates. A total of eight slides were used in this study.

Repositories


GEO

GSE36339

ArrayExpress

E-GEOD-36339

BioProject

PRJNA153299

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Contact


Josep Planas
Josep V Planas