|Application:||Gene expression microarray analysis|
|Number of samples:||8|
|Release date:||Mar 16 2012|
|Last update date:||May 20 2013|
|Dataset link||Transcriptomic response of skeletal muscle to lipopolysaccharide in the gilthead seabream (Sparus aurata)|
Total RNA from pooled control (n = 5) and LPS-treated (n = 5) sea bream white and red muscle tissues was labeled with Cy3-dUTP and Cy5-dUTP (GE Healthcare, Barcelona, Spain). We used a dye swap experimental design and each cDNA from a pooled RNA sample was hybridized to two microarrays. For the first slide, test and control cDNA were labeled with Cy5 and Cy3 respectively, and for the second array dye assignment was reversed. Therefore, samples from individual fish within each group were pooled and expression values shown represent the means of 6 × 2 = 12 technical replicates. A total of eight slides were used in this study.
Josep V Planas