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DGRC specifications

Information


Unique identifier OMICS_22550
Name DGRC
Alternative name Drosophila Genetic Resource Consortium
Restrictions to use None
Community driven No
Data access File download, Browse
User data submission Not allowed
Maintained Yes

Taxon


  • Invertebrates
    • Drosophila melanogaster

DGRC citations

 (20)
library_books

Comparative analyses of co evolving host parasite associations reveal unique gene expression patterns underlying slavemaker raiding and host defensive phenotypes

2018
Sci Rep
PMCID: 5792630
PMID: 29386535
DOI: 10.1038/s41598-018-20262-y

[…] ociated molecular signals, which might have important down-stream affects upon behaviour.For RNA isolation, we followed the protocol of the Center for Genomics and Bioinformatics Bloomington (https://dgrc.bio.indiana.edu/include/file/CGB-TR-200610.pdf). In short, 200 µl chloroform was added to each Trizol sample and the mixture shaken vigorously for 15 seconds, and then centrifuged for 15 min at 4 […]

library_books

Identification of genetic networks that act in the somatic cells of the testis to mediate the developmental program of spermatogenesis

2017
PLoS Genet
PMCID: 5634645
PMID: 28957323
DOI: 10.1371/journal.pgen.1007026

[…] FP.nls (UAS-RedStinger), Baz::GFP (bazCC01941), Dlg1::GFP (dlg1YC0005), DonJuan::GFP (dj-GFP.S), and hh-LacZ (hhP30). Vasa::GFP (vas.EGFP.HA) provided by the Kyoto Drosophila Genetic Resource Center (DGRC). upd-LacZ (upd1-PD) courtesy of David Bilder, University of California Berkeley, USA. bam-GFP (bam-GFP-799/+133) courtesy of Christian Bökel, Center for Regenerative Therapies Dresden, Germany. […]

library_books

Atrophin controls developmental signaling pathways via interactions with Trithorax like

2017
eLife
PMCID: 5409829
PMID: 28327288
DOI: 10.7554/eLife.23084.040

[…] Two step crosslinking ChIP was required in order for ChIP to enrich for positive controls when using the SG2524 anti-Atro antibody (see below). S2 cells (107 cells/mL, S2-DGRC, stock #6 from Drosophila Genomics Research Center) were washed three times in sterile PBS. Washed cells were first fixed with 2 mM ethylene glycol bis(succinimidyl succinate) (EGS) in PBS for 45 […]

library_books

The kinetics of pre mRNA splicing in the Drosophila genome and the influence of gene architecture

2017
eLife
PMCID: 5762160
PMID: 29280736
DOI: 10.7554/eLife.32537.022

[…] Drosophila melanogaster S2 cells (Stock Number #6) were obtained directly from the Drosophila Genomics Resource Center (DGRC) in Bloomington Indiana, and maintained at low passage number as indicated by DGRC guidelines. Cells were regularly confirmed to be free of contamination (e.g. mycoplasma) through PCR-based tests […]

library_books

Evolution, Expression, and Function of Nonneuronal Ligand Gated Chloride Channels in Drosophila melanogaster

2016
PMCID: 4938653
PMID: 27172217
DOI: 10.1534/g3.116.029546

[…] BACPACs were ordered (DGRC), homology arms designed, and recombineering performed as per . The modified BAC fused the GAL4 coding region, in-frame to the start codon and was predicted to express Gal4 in a pattern controlle […]

library_books

Notch stimulates growth by direct regulation of genes involved in the control of glycolysis and the tricarboxylic acid cycle

2016
Open Biol
PMCID: 4772804
PMID: 26887408
DOI: 10.1098/rsob.150155

[…] d for immunostainings were carefully staged, only early L3 larvae that came out of food within a 2 h window were used for dissections. Additional flies used in the paper were HexA–Gal4 enhancer trap (DGRC 105136), H2 (BL517 over TM6 balancer), N55e11 (BL28813). The strength of the signal from the HexA–Gal4 reporter was quantified in ImageJ by calculating the integrated density of HexA immunostaini […]

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