Differential methylation site identification software tools | DNA methylation microarray data analysis
After correct preprocessing of the data (i.e. filtering out problematic probes and normalizing the data), differential methylation analysis can be performed. Generally, the first approach consists in a single-probe analysis. Statistical tests (such as the t-test or Mann–Whitney test) are used, and when the P-values obtained are below a given threshold (e.g. <0.05), the sites are considered as differentially methylated and referred as differentially methylated positions (DMPs). In this way, several researchers have identified numerous DMPs although the absolute difference in methylation of the CpG sites between two groups of samples was small (i.e. below 5% of methylation difference).
A suite of computational tools that incorporate state-of-the-art statistical techniques for the analysis of DNAm data. minfi provides methods for preprocessing, quality assessment and detection of differentially methylated regions from the kilobase to the megabase scale. Several preprocessing algorithms are available and the infrastructure provides a convenient way for developers to easily implement their techniques as Bioconductor tools. By making SNP annotation available, users can choose to be cautious about probes that may behave unexpectedly due to the inclusion of a SNP in the probe sequence. minfi is unique in that it provides both bump hunting and block finding capabilities, and the assessment of statistical significance for the identified regions. Finally, because the package is implemented in Bioconductor, it gives users access to the countless analysis and visualization tools available in R.
Allows Illumina HumanMethylation BeadChip analysis. ChAMP is an integrated analysis pipeline including functions for (i) filtering low quality probes, adjustment for Infinium I and Infinium II probe design, (ii) batch effect correction, detecting differentially methylated positions (DMPs), (iii) finding differentially methylated regions (DMRs) and (iv) detection of copy number aberrations. The software also allows detection of differentially methylated genomic blocks (DMB) and Gene Set Enrichment Analysis (GSEA).
An R package for comprehensive analysis of DNA methylation data obtained with any experimental protocol that provides single-CpG resolution, including Infinium 450K microarray and bisulfite sequencing protocols, but also MeDIP-seq and MBD-seq.
Assists users in investigating normalized omics large datasets. Qlucore Omics Explorer is a modular platform divided into four main functionalities (i) Visualization includes features for generating various plots types including principal component analysis (PCA) plots and real-time visualization; (ii) Exploration furnishes tools for comparing, browsing and selecting targeted information (iii) Analysis includes statistical methods such as quadratic regression, f-tests, or ANOVA and (iv) Sharing allows users to export results as multiple formats including videos or variable lists.
A Windows command-line application that implements a Bayesian wavelet-based functional mixed model methodology for functional data analysis. WFMM can be generally applied to any complex functional data sampled on a fine grid, not just methylation data, and so can be readily applied to other genome-wide data including copy number and tiling transcriptome arrays. The method is computationally intensive, but the software is optimized so that it can handle very large data sets.
An R package that can efficiently perform the statistical analysis needed for increasingly large methylation datasets. CpGassoc can perform standard analyses of large datasets very quickly with no need to impute the data. It can handle mixed effects models with chip or batch entering the model as a random intercept. CpGassoc also includes tools to apply quality control filters, perform permutation tests, and create QQ plots, manhattan plots, and scatterplots for individual CpG sites.