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Diffraction PSF 3D specifications


Unique identifier OMICS_25615
Name Diffraction PSF 3D
Software type Application/Script
Interface Command line interface
Restrictions to use None
Operating system Windows
Programming languages Java
Computer skills Advanced
Stability Stable
Source code URL http://www.optinav.info/download/Diffraction_PSF_3D.java
Maintained Yes




No version available

Additional information


Diffraction PSF 3D citations


Hookworm Secreted Extracellular Vesicles Interact With Host Cells and Prevent Inducible Colitis in Mice

Front Immunol
PMCID: 5936971
PMID: 29760697
DOI: 10.3389/fimmu.2018.00850
call_split See protocol

[…] lsbad, CA, USA) and images were visualized on a laser scanning confocal microscope (Zeiss 780 NLO, Zeiss, Oberkochen, Germany). Confocal image deconvolution was performed in ImageJ using the plugins “Diffraction PSF 3D” for PSF calculation and “DeconvolutionLab” with the Tikhonov–Miller algorithm for 2D deconvolution (). […]


Over elongation of centrioles in cancer promotes centriole amplification and chromosome missegregation

Nat Commun
PMCID: 5871873
PMID: 29593297
DOI: 10.1038/s41467-018-03641-x
call_split See protocol

[…] m number of iterations = 25, terminate = 0.01 (terminate if mean delta less than this value). This methodology requires a normalised point spread function (PSF), which was previously created with the Diffraction PSF 3D PlugIn of ImageJ with the parameters: Index of refraction of the mounting median = 1.46 (ProLong® Gold Antifade Reagent, Molecular Probes®); numerical aperture NA = 1.44, wavelength […]


Characterization of Trichuris muris secreted proteins and extracellular vesicles provides new insights into host–parasite communication

J Extracell Vesicles
PMCID: 5795766
PMID: 29410780
DOI: 10.1080/20013078.2018.1428004

[…] ates (five each). The whole experiment was repeated to perform laser scanning confocal imaging on a 780 NLO microscope (Zeiss). Confocal image deconvolution was performed in ImageJ using the plugins “Diffraction PSF 3D” for PSF calculation and “DeconvolutionLab” with the Richardson–Lucy algorithm for 3D deconvolution and Tikhonov–Miller algorithm for 2D deconvolution []. […]


Coupling of Polo kinase activation to nuclear localization by a bifunctional NLS is required during mitotic entry

Nat Commun
PMCID: 5700101
PMID: 29167465
DOI: 10.1038/s41467-017-01876-8

[…] he same size region in the cytoplasm. Scatter dot plots and statistical analysis were performed using GraphPad Prism 6. For embryo pictures, deconvolution was carried out using the Fiji software and “Diffraction PSF 3D” and “Iterative Deconvolution 3D” plugins. The number of iteration for deconvolution was set to 3. A final projection was made on the z-planes containing the nucleus and the final i […]


Caenorhabditis elegans ATPase inhibitor factor 1 (IF1) MAI 2 preserves the mitochondrial membrane potential (Δψm) and is important to induce germ cell apoptosis

PLoS One
PMCID: 5568743
PMID: 28829773
DOI: 10.1371/journal.pone.0181984

[…] microscope equipped with an AxioCam MRc camera (Zeiss). The pictures were captured using AxioVision software (Zeiss) and deconvolved using ImageJ software [] with Parallel Spectral Deconvolution and Diffraction PSF 3D plugins. Larvae and adult animals expressing MAI-2::GFP and TOMM-20::mCherry, GFP::MAI-1 and MAI-1::mCherry were observed by confocal imaging using an Olympus Fluoview Confocal micr […]


Abnormally Increased Secretion in Olfactory Neuronal Precursors from a Case of Schizophrenia Is Modulated by Melatonin: A Pilot Study

Int J Mol Sci
PMCID: 5535930
PMID: 28703738
DOI: 10.3390/ijms18071439

[…] ImageJ software (version 1.51j8, U.S. National Institutes of Health, Bethesda, MD, USA) and the Iterative Deconvolve 3D plugin (DAMAS3, version 5.2, Bellevue, WA, USA). The PSF was generated with the Diffraction PSF 3D plugin (version 2.0, Bellevue, WA, USA). Images were then converted to 8 bits, background values were substracted, and VAMP1/2-fluorescence values were calculated as the ratio betwe […]


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