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A large (and small) de novo genome assembler. DISCOVAR de novo quickly generates highly accurate and complete assemblies using the same single library data. It requires reads from only a single PCR-free library, and has tested well on relatively homozygous human and mouse genomes. DISCOVAR de novo has the potential to create, from relatively inexpensive sequencing libraries and read coverage, assemblies that are sufficiently complete and contiguous to serve a wide range of downstream comparative, population, and functional genomic analyses.

Software type:
Package
Interface:
Command line interface
Restrictions to use:
Academic users only
Input data:
All that is required are paired end reads, contained within one or more BAM files.
Biological technology:
Illumina
Operating system:
Unix/Linux
License:
Commercial
Computer skills:
Advanced
Stability:
Stable
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Institution(s)

The Broad Institute of MIT and Harvard, Cambridge, MA, USA

Funding source(s)

This project has been funded in part with Federal funds from the National Human Genome Research Institute, National Institutes of Health, Department of Health and Human Services, under grants R01HG003474 and U54HG003067, and in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No. HHSN272200900018C.

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    PMID: 22676195
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    PMID: 24086547
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    PMID: 23358380
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    PMID: 23870653
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    PMID: 22147368
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    PMID: 24930142
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    PMID: 21102452
  • (Love et al., 2016) Evaluation of DISCOVAR de novo using a mosquito sample for cost-effective short-read genome assembly. BMC genomics.
    PMID: 26944054

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