An automatic neuron tracing method. DF-Tracing can automatically trace complicated neuron structures set in noise-contaminated microscopic images. This method first extracts the neurite signal (foreground) from a noisy image by using anisotropic filtering and automated thresholding. Then, DF-Tracing executes a coupled distance-field (DF) algorithm on the extracted foreground neurite signal and reconstructs the neuron morphology automatically.
Key Laboratory of Medical Image Computing, Ministry of Education, Northeastern University, Shenyang, China; Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA; Marine Biological Laboratory, Woods Hole, MA, USA; Allen Institute for Brain Science, Seattle, WA, USA
DF-Tracing funding source(s)
This work was supported by Howard Hughes Medical Institute and by Chinese National Natural Science Foundation under grant No.61001047 and 61172002.