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DNAWorks specifications

Information


Unique identifier OMICS_08705
Name DNAWorks
Interface Web user interface
Restrictions to use None
Computer skills Basic
Version 3.2.2
Stability Stable
Maintained Yes

Publications for DNAWorks

DNAWorks in publications

 (42)
PMCID: 5522410
PMID: 28733633
DOI: 10.1038/s41598-017-06428-0

[…] it is easy to evaluate lycopene production via the detection of red color in e. coli cells.figure 2 , oligos for synthesizing the genes of the lycopene biosynthetic pathway were designed by dnaworks. the gene sequences were split into overlapping fragments using dnaworks and flanked by common primer pairs (fragment-specific primers: fra-f and fra-r in supplementary tableĀ ) […]

PMCID: 5425238
PMID: 28508060
DOI: 10.1126/sciadv.1602426

[…] in any organism where transgenic techniques exist and biotin-phenol can be delivered., the protein sequence of soybean apx with the w41f mutation () was optimized for c. elegans expression using dnaworks to design primers (). primers were annealed using a two-step polymerase chain reaction (pcr) method, and double-stranded dna was cloned into gateway vector pdonr 221 using bp clonase ii […]

PMCID: 5423893
PMID: 28067236
DOI: 10.1038/ncomms14023

[…] interactions will provide insight into their impact on hosts and the constraints on evolution of a minimalistic eukaryotic genome., soybean apx (w41f) was optimized for c. elegans expression using dnaworks to design primers. these primers were annealed using a two-step pcr method and cloned into gateway plasmid pdonr 221. gibson cloning was then used to introduce gfp as an n-terminal fusion, […]

PMCID: 5126662
PMID: 27897254
DOI: 10.1038/srep37869

[…] further increase the expression level of xylanase in p. pastoris, the gene sequence of xynhbn188a was optimized based on the codon usage bias of p. pastoris. the codon-optimized gene was designed by dnaworks (http://helixweb.nih.gov/dnaworks/) and synthesized by genscript (china). primers hbsf and hbsr () were used to amplify xynhbn188as gene and hb3 and hb4 primers () for xynhbn188a. […]

PMCID: 4886644
PMID: 27303654
DOI: 10.4314/ovj.v6i2.1

[…] shown in . this gene was codon-optimized for expression in e.coli. the vector nti suite was used for reverse translation coupled with codon-optimization for heterologous species (e.coli k12)., the dnaworks v3.2.2 was used to calculate sequences of primers for the de novo synthesis of dna fragments. the primers used for the de novo gene synthesis are listed in . these primers were designed […]


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DNAWorks institution(s)
Macromolecular Crystallography Laboratory, National Cancer Institute at Frederick, MD, USA

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