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Protocols

DoBISCUIT specifications

Information


Unique identifier OMICS_09214
Name DoBISCUIT
Alternative name Database of BIoSynthesis clusters CUrated and InTegrated
Restrictions to use None
Maintained Yes

Maintainer


  • person_outline Nobuyuki Fujita

Publication for Database of BIoSynthesis clusters CUrated and InTegrated

DoBISCUIT citations

 (4)
library_books

The plastid genome of some eustigmatophyte algae harbours a bacteria derived six gene cluster for biosynthesis of a novel secondary metabolite

2016
Open Biol
PMCID: 5133447
PMID: 27906133
DOI: 10.1098/rsob.160249

[…] gene of the novel operon analysed here has been experimentally characterized in any species and, hence, no formal name has been proposed for any of the genes. Furthermore, the operon is not listed in DoBISCUIT, a manually curated and regularly updated database of secondary metabolite biosynthetic gene clusters (http://www.bio.nite.go.jp/pks/ []). To facilitate communication about the new operon, w […]

call_split

Genome guided Investigation of Antibiotic Substances produced by Allosalinactinospora lopnorensis CA15 2T from Lop Nor region, China

2016
Sci Rep
PMCID: 4749953
PMID: 26864220
DOI: 10.1038/srep20667
call_split See protocol

[…] e other hand, in order to assess the antibiotics-producing capacity of A. lopnorensis CA15-2T, 15 gene clusters involved in synthesizing the known diverse antibiotics were randomly extracted from the DOBISCUIT database () to be compared with the genes of A. lopnorensis CA15-2T using BLAST, respectively. Furthermore, 10 gene sets from 10 known antibiotic-producing bacteria, which were picked up acc […]

library_books

Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii

2015
Appl Environ Microbiol
PMCID: 4551226
PMID: 26092459
DOI: 10.1128/AEM.01039-15

[…] rmed using NCBI package 2.2.26+ () and SequenceServer () or prfectBLAST (). Automatic primer design was carried out using the Primer3Plus web server (). DoBISCUIT (http://www.bio.nite.go.jp/pks/top) (Database of Biosynthesis clusters Curated and Integrated) () was used to obtain curated and annotated versions of ansamycin-related gene clusters. The sequence of phage P1-derived artificial chromosom […]

call_split

IMG ABC: A Knowledge Base To Fuel Discovery of Biosynthetic Gene Clusters and Novel Secondary Metabolites

2015
MBio
PMCID: 4502231
PMID: 26173699
DOI: 10.1128/mBio.00932-15
call_split See protocol

[…] gene clusters. GenBank records with sequences longer than 3,000 bp were retained and manually curated for their encoded SM name, whenever possible. BCs were also retrieved from ClusterMine360 () and DoBiscuit () irrespective of their length and reconciled with those retrieved from the NCBI database in order to produce a nonredundant list of BCs. The resulting BCs were mapped to isolate genomes an […]


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DoBISCUIT institution(s)
Biological Resource Center, National Institute of Technology and Evaluation (NBRC), Shibuya-ku, Tokyo, Japan

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