Digital PCR is a new technology that follows the concept of traditional PCR but allows absolute quantification of a number of nucleic acid copies without requirement for gel electrophoresis. In dPCR, a sample is partitioned into droplets containing target sequence and fluorophores, which are then counted as positive or negative. Poisson law of small numbers enable to estimate precisely the number of copies in the sample.
Computes copies per droplet (CPDs) for multiplexed ddPCR reactions. ddPCRclust first finds the centroids and labelled them. Then, the application allocates the rain and determines the number of positive droplets to lastly determines the CPDs. It accepts up to four targets in a non-orthogonal arrangement. The package can be executed as a command-line system or through a graphic interface by using ddPCRvis module.
Quantifies DNA which holds great promise in clinical diagnostics. ddpcr allows users to analyze ddPCR data using an interactive graphical interface. It includes an interactive web application with a visual user interface to facilitate analysis for anyone who is not comfortable with using R. The counting of droplets emitting a sequence-specific fluorescent signal permits the number of copies of that sequence present in the sample to be quantified with excellent sensitivity and precision.
Classifies droplets tacked from Bio-Rad's QuantaSoft. Twoddpcr generates a summary of the positive/negative droplet counts which can then be used to estimate the number of molecules using the Poisson distribution. It facilitates the automatic classification of general two channel ddPCR data. The tool could be useful to see the droplet counts for each class in addition to the overall droplet count at a glance.
It is used for the analysis of data derived from the QuantStudio 3D Digital PCR instrument. QuantStudio 3D AnalysisSuite Cloud Software is an easy-to-use data analysis tool with an intuitive user interface that allows multiple applications such as pathogen, viral load, and copy number detection.
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