E-CRISP statistics

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E-CRISP specifications


Unique identifier OMICS_03718
Interface Web user interface
Restrictions to use None
Input data Gene name, target region seq (single and batched)
Computer skills Basic
Version 4.2
Stability Stable
Maintained Yes


  • person_outline Florian Heigwer <>

Publication for E-CRISP

E-CRISP in publications

PMCID: 5916876
PMID: 29695804
DOI: 10.1038/s41598-018-24690-8

[…] the chances of regenerating successfully edited plants, designing highly specific and effective grnas is important. multiple bioinformatics tools, such as wheatcrispr (cram et al., submitted), e-crisp and crisprdirect, have been developed to facilitate the design of grnas targeting specific loci in the wheat genome and prediction of off-target sites. among these, the wheatcrispr tool […]

PMCID: 5951413
PMID: 29770349
DOI: 10.21769/BioProtoc.2765

[…] (bio-rad laboratories, catalog number: 1640302), autoclave, geneious v8.0 () or higher, to design grna and repair template (replacement gene). other grna design software can be used as well, such as e-crisp (), blat (), design two guide rna (grna) sequences targeting the open reading frame (orf) for the yeast gene to be replaced using geneious, or a similar tool such as e-crisp ()., grna […]

PMCID: 5856429
PMID: 29547664
DOI: 10.1371/journal.pone.0194451

[…] to lmp-1 for in vivo recombination with lmp-1::rfpunc-54., all sgrnas were designed by using either the online sgrna design tool http://crispr.cos.uni-heidelberg.de/index.html [] or http://www.e-crisp.org/e-crisp/ []. sgrna expression plasmids were cloned by using prb1017 following the protocol of []. the sgrnas were expressed under the control of a pu6 promoter. in order to obtain large […]

PMCID: 5838027
PMID: 29508097
DOI: 10.1186/s13568-018-0564-9

[…] system developed by stovicek et al. () was used for selective targeting of sites. the grna target sequence for gre3 was selected using the crispy tool (ronda et al. ) and for xyld using the e-crisp tool (heigwer et al. ). the grna plasmids plwa25 and plwa36 were constructed by amplifying the grna backbone as described by stovicek et al. () using a phosphorylated forward primer, 102 […]

PMCID: 5820354
PMID: 29503817
DOI: 10.3389/fcell.2018.00014

[…] 1 mm gyy4137 or 1 mm nahs at 6 hpf. all embryos were then developed to 30 or 52 hpf in the 28°c incubator. pictures were taken as described earlier., crispr/cas9 target sequences were designed using e-crisp. (heigwer et al., ). pt3ts-ncas9n was a gift from wenbiao chen (addgene plasmid # 46757) (jao et al., ). sgrna and capped cas9 mrna were synthesized by in-vitro transcription as previously […]

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E-CRISP institution(s)
Division of Signaling and Functional Genomics, German Cancer Research Center (DKFZ), Heidelberg, Germany; Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Heidelberg, Germany

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