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Protocols

E-CRISP specifications

Information


Unique identifier OMICS_03718
Name E-CRISP
Interface Web user interface
Restrictions to use None
Input data Gene name, target region seq (single and batched)
Computer skills Basic
Version 4.2
Stability Stable
Maintained Yes

Maintainer


  • person_outline Florian Heigwer

Publication for E-CRISP

E-CRISP citations

 (72)
library_books

Targeted mutagenesis in wheat microspores using CRISPR/Cas9

2018
Sci Rep
PMCID: 5916876
PMID: 29695804
DOI: 10.1038/s41598-018-24690-8

[…] mprove the chances of regenerating successfully edited plants, designing highly specific and effective gRNAs is important. Multiple bioinformatics tools, such as WheatCRISPR (Cram et al., submitted), E-CRISP and CRISPRdirect, have been developed to facilitate the design of gRNAs targeting specific loci in the wheat genome and prediction of off-target sites. Among these, the WheatCRISPR tool offers […]

library_books

Accurate lattice parameters from 2D periodic images for subsequent Bravais lattice type assignments

2018
PMCID: 5871643
PMID: 29607290
DOI: 10.1186/s40679-018-0051-z

[…] the three computer programs is in order in this final section of this review.Of the three tested programs, the one that has been around for more than a quarter of a century as a windows executable, i.e., CRISP, performed best. The CRISP program is also the only one of the three tested programs that allows for a direct route to the extraction of the parameters of rectangular centered Bravais lattic […]

library_books

Single step Precision Genome Editing in Yeast Using CRISPR Cas9

2018
PMCID: 5951413
PMID: 29770349
DOI: 10.21769/BioProtoc.2765

[…] Geneious v8.0 () or higher, to design gRNA and repair template (replacement gene). Other gRNA design software can be used as well, such as E-CRISP ()BLAT () […]

library_books

Overlapping expression patterns and functions of three paralogous P5B ATPases in Caenorhabditis elegans

2018
PLoS One
PMCID: 5856429
PMID: 29547664
DOI: 10.1371/journal.pone.0194451

[…] All sgRNAs were designed by using either the online sgRNA design tool http://crispr.cos.uni-heidelberg.de/index.html [] or http://www.e-crisp.org/E-CRISP/ []. sgRNA expression plasmids were cloned by using pRB1017 following the protocol of []. The sgRNAs were expressed under the control of a PU6 promoter. In order to obtain large ge […]

library_books

Exploring d xylose oxidation in Saccharomyces cerevisiae through the Weimberg pathway

2018
PMCID: 5838027
PMID: 29508097
DOI: 10.1186/s13568-018-0564-9

[…] ISPR–Cas9 system developed by Stovicek et al. () was used for selective targeting of sites. The gRNA target sequence for GRE3 was selected using the CRISPy tool (Ronda et al. ) and for xylD using the E-CRISP tool (Heigwer et al. ). The gRNA plasmids pLWA25 and pLWA36 were constructed by amplifying the gRNA backbone as described by Stovicek et al. () using a phosphorylated forward primer, 102 for p […]

library_books

Cystathionine β Synthase Is Necessary for Axis Development in Vivo

2018
PMCID: 5820354
PMID: 29503817
DOI: 10.3389/fcell.2018.00014

[…] CRISPR/CAS9 target sequences were designed using E-CRISP. (Heigwer et al., ). pT3TS-nCas9n was a gift from Wenbiao Chen (Addgene plasmid # 46757) (Jao et al., ). sgRNA and capped cas9 mRNA were synthesized by in-vitro transcription as previously des […]

Citations

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E-CRISP institution(s)
Division of Signaling and Functional Genomics, German Cancer Research Center (DKFZ), Heidelberg, Germany; Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Heidelberg, Germany

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