e-PCR specifications

Information


Unique identifier OMICS_02345
Name e-PCR
Alternative name Electronic PCR
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability No
Maintained No

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Publication for Electronic PCR

e-PCR in publications

 (108)
PMCID: 5916874
PMID: 29707045
DOI: 10.7150/jgen.22460

[…] of reads. similar analysis were performed for forward primer only of pair, 3 prime end of primer pairs and 3 prime end only of forward primer., for in-silico testing of designed primer pairs, e-pcr tool standalone version was employed {ref e-pcr}. for all primer pairs of each serotype which were given as query set, two separate searches were performed to two different genome data sets. […]

PMCID: 5940161
PMID: 29555822
DOI: 10.1534/g3.118.200134

[…] dna was extracted after 3 days without selection. genomic regions spanning the target sites were amplified by pcr for sequencing. sanger sequencing chromatograms for both the blos2 and yellow-e pcr products showed a set of c/t peaks in the target sites, indicating that the intended base substitutions did occur (). to confirm the base substitutions, the pcr products were cloned […]

PMCID: 5863996
PMID: 29513800
DOI: 10.1590/0102-672020180001e1339

[…] indivíduos histopatologicamente confirmados com aa em comparação com 249 controles que foram submetidos à tomografia computadorizada abdominal (tc). dados clínicos e epidemiológicos, valores de imc e pcr foram extraídos de registros médicos. as tc foram avaliadas para achados de aa e medidas de composição corporal. , os valores da pcr aumentaram de acordo com o imc dos pacientes, com […]

PMCID: 5836569
PMID: 29511604
DOI: 10.7717/peerj.4266

[…] ssr regions that were deposited in genbank, using primer-blast (). detailed primer sequences and their sources are listed in . pcr was conducted in 20 ul reaction mixtures containing 1 × nexpro™ e pcr master mix (genes laboratories, bokjeong-dong, south korea), 0.2 µm each of the forward and reverse primers, and approximately 20 ng of genomic dna. the designed primers were initially tested […]

PMCID: 5751396
PMID: 29297325
DOI: 10.1186/s12870-017-1193-1

[…] treatment to increase the reproducibility and proportion of long fragments. size selection using caliper labchip xt (perkin-elmer) was carried out to obtain library inserts 250–300 bp long. e-pcr, enrichment and quantification for ion torrent sequencing were performed with one-touch 2 and one-touch es systems (life technologies). sequencing was carried out on the ion pgm (life […]


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e-PCR institution(s)
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA

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