e-PCR pipeline

e-PCR specifications

Information


Unique identifier OMICS_02345
Name e-PCR
Alternative name Electronic PCR
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability No
Maintained No

Publication for Electronic PCR

e-PCR IN pipeline

2014
PMCID: 4014442
PMID: 24810276
DOI: 10.1371/journal.pgen.1004281

[…] from coding-exon sequences (cds) using primer3 [100] (opt. amplicon 200 bp, primer 18–25 bp, opt. tm 60°c, max. δtm 1°c, min. gc clamp 2 bp, max. homopolymer 3 bp). in silico pcr screening via e-pcr [101] required ≤1 amplicon (10 bp to 10 kbp) versus genome assembly and cds sequences. quantitative pcr was performed with 2 technical replicates and 3 biological replicates. log2 fold-changes […]

e-PCR institution(s)
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA

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