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Protocols

e-PCR specifications

Information


Unique identifier OMICS_02345
Name e-PCR
Alternative name Electronic PCR
Interface Web user interface
Restrictions to use None
Computer skills Basic
Stability No
Maintained No

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Publication for Electronic PCR

e-PCR citations

 (386)
library_books

Microfluidic PCR Amplification and MiSeq Amplicon Sequencing Techniques for High Throughput Detection and Genotyping of Human Pathogenic RNA Viruses in Human Feces, Sewage, and Oysters

2018
Front Microbiol
PMCID: 5934477
PMID: 29755444
DOI: 10.3389/fmicb.2018.00830

[…] t human pathogenic viruses in sewage (; ), activated sludge (; ), and reclaimed water (). A main advantage of metagenomic approaches is that they avoid bias introduced by PCR amplification of cDNA; i.e., PCR-based approaches require the use of oligonucleotide primers, with the consequence that viral populations with primer–template mismatches are overlooked. On the contrary, the population of huma […]

library_books

Redundancy in Anaerobic Digestion Microbiomes during Disturbances by the Antibiotic Monensin

2018
Appl Environ Microbiol
PMCID: 5930344
PMID: 29500266
DOI: 10.1128/AEM.02692-17

[…] tion kit (MoBio, Carlsbad, CA, USA). DNA extracted from the cow hindgut biomass samples was sent to the Earth Microbiome Project at the University of Colorado Boulder for further sample processing (i.e., PCR amplification via universal primers targeting the V4 region of the 16S rRNA gene—515F forward primer and 806R reverse primer—amplicon cleanup, and Illumina HiSeq 2000 sequencing). Details of t […]

library_books

In silico Designing and Testing of Primers for Sanger Genome Sequencing of Dengue Virus Types of Asian Origin

2018
J Genomics
PMCID: 5916874
PMID: 29707045
DOI: 10.7150/jgen.22460

[…] For In-silico testing of designed primer pairs, e-PCR tool standalone version was employed {Ref e-PCR}. For all primer pairs of each serotype which were given as query set, two separate searches were performed to two different genome data sets. Fir […]

library_books

Impact of F1Fo ATP synthase dimer assembly factors on mitochondrial function and organismic aging

2018
PMCID: 5878687
PMID: 29610761
DOI: 10.15698/mic2018.04.625

[…] nt-Kit (Macherey-Nagel). Reverse transcription of 1 mg total RNA was performed with the RevertAid™ M-MuLV reverse transcriptase (ThermoScientific) according to the manufacturer’s instruction. Real-time PCR was realized using iQ SYBR Green Supermix (BioRad) followed by the manufacturer’s protocol. For each gene, the efficiency (E) of the primer pairs was calculated based on a real-time PCR with a d […]

library_books

Programmable Single and Multiplex Base Editing in Bombyx mori Using RNA Guided Cytidine Deaminases

2018
PMCID: 5940161
PMID: 29555822
DOI: 10.1534/g3.118.200134

[…] genomic DNA was extracted after 3 days without selection. Genomic regions spanning the target sites were amplified by PCR for sequencing. Sanger sequencing chromatograms for both the Blos2 and Yellow-e PCR products showed a set of C/T peaks in the target sites, indicating that the intended base substitutions did occur (). To confirm the base substitutions, the PCR products were cloned into the pEA […]

library_books

Development and evaluation of a culture free microbiota profiling platform (MYcrobiota) for clinical diagnostics

2018
PMCID: 5948305
PMID: 29549470
DOI: 10.1007/s10096-018-3220-z

[…] a priori knowledge of the potential pathogen before a test is performed. To overcome these limitations, the bacterial composition can be defined and genera identified using a culture-free, broad-range PCR strategy that targets the prokaryotic 16S rRNA gene followed by next-generation sequencing (NGS) []. However, to date, 16S rRNA gene NGS methods to profile microbial compositions have been focus […]


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e-PCR institution(s)
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA

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