E-TALEN statistics

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Associated diseases

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E-TALEN specifications

Information


Unique identifier OMICS_04702
Name E-TALEN
Interface Web user interface
Restrictions to use None
Input data A file in or a new line separated list of gene symbols and/or accession numbers.
Input format FASTA
Computer skills Basic
Stability Stable
Maintained Yes

Documentation


Maintainers


  • person_outline Michael Boutros <>
  • person_outline Florian Heigwer <>

Additional information


http://b110-wiki.dkfz.de/confluence/display/TALEN//E-TALEN+wiki

Publication for E-TALEN

E-TALEN in publications

 (6)
PMCID: 5593860
PMID: 28894087
DOI: 10.1038/s41467-017-00530-7

[…] plasmids were transfected into hcc cells and selected with puromycin for 7–14 days. hif-1α-stable ko hcc cell lines were established by talen approach. hif-1α-specific tale domains were designed by e-talen software, assembled and cloned into ptalen v2 vector by “tale toolbox”. hif-1α ko construct was transfected into mhcc97l hcc cells, respectively. stable cells were clonally expanded. […]

PMCID: 5511212
PMID: 28710417
DOI: 10.1038/s41598-017-05696-0

[…] 10% was shown in hela cell line where a complete abrogation of e7 expression was observed. tools used to design talens aid researchers in targeting specific regions of the gene. while talen-t, e-talen and mojo hand design talens based on genomic context, restriction enzymes and off- target effects, sapta predicts talen activity based on rvd composition and site length, restriction enzymes. […]

PMCID: 5034111
PMID: 27502025
DOI: 10.1038/cr.2016.93

[…] was used as a control. cells were collected for further analysis at 72 h post electroporation., talen target sites were designed and constructed according to the instruction provided with the e-talen kit (viewsolid biotech). briefly, units were assembled to construct the tale repeats according to the target sequences, using several rounds of digestion/ligation. the constructed repetitive […]

PMCID: 4939295
PMID: 27462304
DOI: 10.3389/fmicb.2016.01067

[…] usa). all primers used in this study are listed in table ., primers f1 and r1 were used to obtain the full sequence of adh2. the 17 bp target sequence of agttggagcataaggat in adh2 was selected using e-talen. the left and right arms of sequence-specific talens (ptalen l48 and ptalen r36) targeting 17 bp sequences adjacent to agttggagcataaggat were constructed through one-step ligation using […]

PMCID: 4786925
PMID: 26750397
DOI: 10.1038/mt.2016.1

[…] at the activity of 96 talen pairs determined that 12 pairs had no activity and 43 pairs had activities below 20% in a model cell line. some talen design tools incorporate ranking of talen pairs. the e-talen webtool incorporates a scoring algorithm for ranking potential talens, but this scoring system was not experimentally validated. the second-generation talen design tool sapta (scoring […]


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E-TALEN institution(s)
German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics and Heidelberg University, Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg, Germany
E-TALEN funding source(s)
Supported by an ERC Advanced Grant of the European Commission.

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