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Protocols

E-TALEN specifications

Information


Unique identifier OMICS_04702
Name E-TALEN
Interface Web user interface
Restrictions to use None
Input data A file in or a new line separated list of gene symbols and/or accession numbers.
Input format FASTA
Computer skills Basic
Stability Stable
Maintained Yes

Documentation


Maintainers


  • person_outline Michael Boutros
  • person_outline Florian Heigwer

Additional information


http://b110-wiki.dkfz.de/confluence/display/TALEN//E-TALEN+wiki

Publication for E-TALEN

E-TALEN citations

 (8)
library_books

Hypoxia inducible factor HIF 1 promotes myeloid derived suppressor cells accumulation through ENTPD2/CD39L1 in hepatocellular carcinoma

2017
Nat Commun
PMCID: 5593860
PMID: 28894087
DOI: 10.1038/s41467-017-00530-7

[…] O plasmids were transfected into HCC cells and selected with puromycin for 7–14 days. HIF-1α-stable KO HCC cell lines were established by TALEN approach. HIF-1α-specific TALE domains were designed by E-TALEN software, assembled and cloned into pTALEN v2 vector by “TALE Toolbox”. HIF-1α KO construct was transfected into MHCC97L HCC cells, respectively. Stable cells were clonally expanded. Frame-shi […]

library_books

TALEN based HPV E7 editing triggers necrotic cell death in cervical cancer cells

2017
Sci Rep
PMCID: 5511212
PMID: 28710417
DOI: 10.1038/s41598-017-05696-0

[…] Cervical cancer is one of the most common cancers in women worldwide and in more than 90% of the cases HPV has been found as the principal etiological agent. We therefore found it relevant to use TALEN-based genome editing tool, to disrupt viral genes in the genome, as an alternate therapeutic approach. TALEN targeting E7 showed good activity in our study. Our results showed nearly 10% editi […]

call_split

TALEN based generation of a cynomolgus monkey disease model for human microcephaly

2016
Cell Res
PMCID: 5034111
PMID: 27502025
DOI: 10.1038/cr.2016.93
call_split See protocol

[…] TALEN target sites were designed and constructed according to the instruction provided with the e-TALEN kit (Viewsolid Biotech). Briefly, units were assembled to construct the TALE repeats according to the target sequences, using several rounds of digestion/ligation. The constructed repetitive T […]

library_books

Improvement of Ethanol Production in Saccharomyces cerevisiae by High Efficient Disruption of the ADH2 Gene Using a Novel Recombinant TALEN Vector

2016
Front Microbiol
PMCID: 4939295
PMID: 27462304
DOI: 10.3389/fmicb.2016.01067

[…] Primers F1 and R1 were used to obtain the full sequence of ADH2. The 17 bp target sequence of AGTTGGAGCATAAGGAT in ADH2 was selected using E-TALEN. The left and right arms of sequence-specific TALENs (Ptalen L48 and Ptalen R36) targeting 17 bp sequences adjacent to AGTTGGAGCATAAGGAT were constructed through one-step ligation using the Fa […]

library_books

Nuclease Target Site Selection for Maximizing On target Activity and Minimizing Off target Effects in Genome Editing

2016
PMCID: 4786925
PMID: 26750397
DOI: 10.1038/mt.2016.1

[…] many potential target sites.,,,,, Despite the ability of well-designed nucleases to target defined loci with high efficiency, the widespread use of TALENs has been hampered by poor performance of some TALEN pairs designed, thereby necessitating the screening of a large number of candidates to find a validated TALEN pair with a high level of activity. For example, a high-throughput study that look […]

library_books

TALEN Based Mutagenesis of Lipoxygenase LOX3 Enhances the Storage Tolerance of Rice (Oryza sativa) Seeds

2015
PLoS One
PMCID: 4671593
PMID: 26641666
DOI: 10.1371/journal.pone.0143877

[…] ous genes were constructed through Golden Gate assembly. The plasmids for TALEN assembly were obtained from www.Addgene.org. The LIC method was modified to allow the incorporation of DNA fragments (i.e., TALEN cassettes) into the destination vectors []. Briefly, a reaction mixture was prepared on ice in a 20 μl volume containing a DNA fragment (400–500 ng), 1×NEB2 buffer (NEB), 0.1 mg/ml BSA (NEB) […]


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E-TALEN institution(s)
German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics and Heidelberg University, Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg, Germany
E-TALEN funding source(s)
Supported by an ERC Advanced Grant of the European Commission.

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