|Application:||Gene expression microarray analysis|
|Number of samples:||6|
|Release date:||Jun 22 2009|
|Last update date:||Oct 29 2018|
|Chemicals:||Adrenal Cortex Hormones, Dexamethasone, Steroids|
|Genes:||TNF, IL1A, IL1B, IL6, CXCL1, CXCL2, CXCL3, CXCL8, CCL3, CCL4, CCL20, CCL24, EGR1, DUSP2, TNFAIP3|
|Dataset link||Corticosteroid Resistant Asthma Is Associated with Classical Activation Of Airway Macrophages and Exposure To LPS|
Patients with a diagnosis of asthma according to American Thoracic Society criteria were selected for evaluation. Asthmatics had a baseline FEV1% predicted of 55% to 85% of predicted, a beta2-adrenergic response of ≥12% of baseline FEV1% predicted and/or a methacholine PC20 value of ≤8 mg/ml. Asthma patients were further subdivided in CR and CS groups, based on their response to steroids. The definition was based on change in FEV1 % predicted over a one-week course of 40 mg/day of oral prednisone. Asthmatic patients were defined as CS if they had an increase in FEV1% predicted of greater than 15% after a one-week course of prednisone, and as CR if less than 12% change in FEV1% predicted was observed. None of the asthmatic subjects recruited for this study had evidence for other types of lung diseases. None of the subjects had received systemic corticosteroid therapy for at least one month prior to bronchoscopy. All subjects provided written informed consent to participate in the study. The study was approved by the Institutional Review Board at National Jewish Medical and Research Center, Denver, CO. Gene array studies of BAL macrophages were performed in 3 CR and 3 CS asthmatics.