Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 54
Release date: Apr 29 2015
Last update date: Nov 22 2016
Access: Public
Taxon: Glycine max
Dataset link Identification of differentially expressed genes between developing seeds of different soybean cultivars

Experimental Protocol


The soybean plants of two soybean varieties Minsoy and Archer and two recombinant inbred lines from the cross that are similar in maturity but differ in yield were grown in St Paul, Minnesota during the summers of 2007 and 2008. In 2007, each line was planted as a single row. In 2008, a randomized complete block (RCB) design was used and each line had 3 replicates planted 1-2 weeks apart. Within each replicate, two rows per line were planted. Seeds were harvested at three developmental stages, namely, seed length = 2 mm, 3.5 mm, and 5-6 mm, which correspond approximately to soybean reproductive stages R4, R5 and early R6, respectively. In 2007 three independent samples were collected for each line and developmental stage. In 2008, two seed samples (one from each row) were collected for each line at each stage within each replicate. The pairs of seed samples were then pooled. Thus, three sets of independent tissue samples were collected for RNA extraction and hybridization on Affymetrix microarrys.

Repositories


GEO

GSE21598

ArrayExpress

E-GEOD-21598

BioProject

PRJNA127135

Download


Contact


Susan Gibson
Susan I Gibson

Dataset Statistics

info

Citations per year

Dataset publication