Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 169
Release date: Mar 30 2010
Last update date: Jan 17 2017
Access: Public
Diseases: Bronchial Diseases, Influenza, Human
Dataset link Dynamic responses of primary human bronchial epithelial cells to influenza virus, viral RNA and interferon-beta

Experimental Protocol


HBECs were stimulated with a 15 minute pulse of 1000U/ml IFNß (PBL, Piscataway, NJ), 100ng/ml vRNA (purified directly from PR8 virus) with LTX transfection reagent (Invitrogen; Carlsbad, California), wild type H1N1 influenza (A/PR/8/34) or ?NS1 virus (PR8 with a deleted NS1 gene, gift from Dr. Garcia-Sastre). Viruses were used at a multiplicity of infection (moi) of 5. Control samples were incubated with media or LTX under the same conditions. Cells were washed, supplemented with warm media and harvested at 11 timepoints (0, .25, .5, 1, 1.5, 2, 4, 6, 8, 12, and 18 hours post-treatment). HBECs were seeded in 6 well plates at a concentration of 250,000/well 18 hours prior to stimultaion. Cells were stimulated with a 15 minute pulse of IFNb, vRNA, infected with PR8 influenza or NS1 deleted influenza, or mock treated

Repositories


GEO

GSE19392

ArrayExpress

E-GEOD-19392

BioProject

PRJNA121751

Download


Contact


Nir Hacohen