Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 1
Release date: Dec 31 2016
Last update date: Jan 1 2017
Access: Public
Chemicals: Amino Acids, Taurine
Dataset link Transcriptome profiling of peripheral blood mononuclear cells from Bos indicus Malnad Gidda breed

Experimental Protocol


Total RNA was isolated using RNeasy Mini kit (Qiagen, Venlo, The Netherlands). RNA was quantitated by 260 nm absorbance with a Nanodrop ND-1000 spectrophotometer (NanoDrop Technologies Inc, DE, USA) as well as by measuring fluorescence using Qubit 2.0 (Life Technologies, NY, USA). The quality of RNA was further determined using capillary electrophoresis with an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). RNA sample was rRNA depleted using Epicentre Ribo-Zero™ kit to enrich the sample for mRNA and non-coding RNA. Enriched RNA samples is further processed to generate RNA library using the TruSeq RNA Sample Preparation v2 kit following the manufactures guide. Paired-end 100bp read sequencing of RNA library was carried out on Illumina HiSeq 2000 platform.

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Ramesha Kerekoppa