Computational protocol: Pulmonary exposure to carbon black nanoparticles and vascular effects

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Protocol publication

[…] The aorta extending from the heart to the iliac arteries from each of the aged mice was cut longitudinally and placed with the intimal surface facing upwards on an objective glass with a few drops of PSS. The aorta was then carefully flattened by adding a cover slip while avoiding any folds or overlap of the vascular tissue. Digital images were obtained of the intimal surface of the aorta by means of an Olympus SZX7 stereo microscope and an Olympus Color View I camera. The level of atherosclerosis in the aorta was measured on the digital images with the public domain Java image processing program ImageJ. [...] The data on vasomotor function were analyzed by ANOVA with unequal variance, as the exposure groups did not exhibit homogeneity of variance. The curve fittings were carried out in GraphPad Prism 4 and the Emax and EC50 with corresponding 95% confidence intervals (CI) were calculated. Differences between groups with non-overlapping 95% CI were considered statistically significant at P < 0.05 level. The data on plaque progression, expression of adhesion molecules and presence of 3-nitrotyrosine, and expression of mRNA were assessed by non-parametric one-way ANOVA (Mann-Whitney U-test or Kruskall-Wallis test) with post-hoc Tukey-type multiple comparison test for effects showing statistical significance in the overall ANOVA test. Statistical significances were tested at P < 0.05 level. The differences in relative precontraction tension between exposure groups were analyzed by One-Way ANOVA (24 hours) or Two-Sample t-Test (2 hours and 24+2 hours). The association between relative precontraction tension and the maximal acetylcholine-induced vasorelaxation was assessed by Spearman correlation. The statistical analyses were performed in Statistica version 5.5 (StatSoft Inc., Tulsa, OK, USA) and SAS version 9.2 (SAS Institute Inc., Cary, NC, USA). […]

Pipeline specifications

Software tools ImageJ, Statistica
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Mus musculus
Diseases Cardiovascular Diseases, Pneumonia, Atherosclerosis
Chemicals Acetylcholine, Phenylephrine, Soot