Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 18
Release date: Apr 1 2012
Last update date: Apr 6 2016
Access: Public
Taxon: Bacillus subtilis
Dataset link Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis

Experimental Protocol


Cells were grown under vigorous agitation at 37 °C in a defined medium (Stülke et al., 1993, J Gen Microbiol 139, 2041-2045). Samples were taken at OD500 0.4 and 1h upon entry into stationary phase. Microarray hybridizations were performed with RNA from three biological replicates. The individual samples were labeled with Cy5; a reference pool containing equal amounts of RNA from all 10 samples was labeled with Cy3.

Repositories


GEO

GSE31249

ArrayExpress

E-GEOD-31249

BioProject

PRJNA146059

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Contact


Ulrike Mäder
Ulrike Mäder