Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 15
Release date: Oct 6 2015
Last update date: Jun 12 2017
Access: Public
Genes: STK38, NDR1
Dataset link A highly conserved bacterial effector localizes to the host plasma membrane and downregulates the expression of the NDR/HIN1-like 13 (NHL13) gene requiried for antibacterial immunity in Arabidopsis

Experimental Protocol


Leaves of 5-week-old Arabidopsis Col-0 plants were vacuum-infiltrated with Pst DC3000, the avrE mutant, the hopM1 mutant or the avrE hopM1 double mutant at 1x108 cfu/ml. Tissue samples were collected by snap-freezing in liquid nitrogen 7 h after bacterial infiltration and RNA was extracted using RNeasy plant mini kits (Qiagen). The Gene Chip Arabidopsis ATH1 genome array (Affymetrix) was used for microarray analysis in the Research Technology Support Facility at Michigan State University.

Repositories


GEO

GSE67179

BioProject

PRJNA279184

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Contact


Sheng Yang He