Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 16
Release date: Nov 21 2017
Last update date: Nov 20 2018
Access: Public
Taxon: Mus musculus
Dataset link RNA-seq of mouse myeloid progenitors reveals two independent pathways for monocyte production via GMPs and MDPs

Experimental Protocol


RNA-seq of myeloid progenitors and Ly6Chi monocytes from mouse bone marrow. 4 progenitor fractions (GMPs, MDPs, GPs and a mixed fraction of MPs + cMoPs) were isolated from the pooled bone marrow of 20 mice. GMPs and MDPs were also cultured in vitro and the monocyte-committed progenitors and Ly6Chi monocytes they produced were also harvested. RNA was extracted from the 4 ex vivo progenitor fractions, and the 4 populations derived in vitro (GMP-derived monocyte progenitors = MP; MDP-derived monocyte progenitors = cMoP; GMP-derived Ly6Chi monocytes = G-mono; MDP-derived Ly6Chi monocytes = M-mono). The whole process was repeated using 20 additional mice to obtain a replicate set of samples.

Repositories


GEO

GSE88982

ENA

SRP091858

BioProject

PRJNA349460

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Contact


Helen Goodridge