Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 24
Release date: Dec 31 2011
Last update date: Feb 22 2018
Access: Public
Taxon: Homo sapiens
Dataset link Interlaboratory study to determine the reproducibility of toxicogenomics datasets

Experimental Protocol


The aim of this study was to evaluate the inter- and intra-laboratory reproducibility of toxicogenomics data for toxicity testing in a regulatory setting. 3 Test Centres performed the same experimental protocol treating HepG2 cells with 3 mM Benzo[a]pyrene (B[a]P) (in 0.5% DMSO) for 24 hours prior to RNA extraction and microarray analysis. Three biological replicates of B[a]P and DMSO treatments were analysed by microarray analysis in each Test Centre. Two Test Centres analysed one sample in triplicate to determine technical reproducibility of the chosen array platform. One Centre analysed two samples in duplicate. 24 samples in total were used in this study

Repositories


GEO

GSE25936

ArrayExpress

E-GEOD-25936

BioProject

PRJNA135687

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Contact


Yeyejide Adeleye
Yeyejide A Adeleye