Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 18
Release date: Dec 22 2015
Last update date: Oct 10 2018
Access: Public
Genes: BMP1, BMP4
Computational protocol: CFX Manager, Bowtie, TopHat, Cufflinks, Enrichr, Hclust, GSEA
Dataset link AGM hematopoietic stem cells are differentially regulated by BMP and Hedgehog signalling pathways

Experimental Protocol


Embryonic day 11 AGM are dissected and either analyzed directly, or after explant culture in conditions containing BMP/Hedgehog with or without cyclopamine. EC: endothelial enriched (CD31+Kit-); MC: mesenchymal cell enriched (CD31-Kit-); HPSC: hematopoietic progenitor/stem cell enriched; AGM11: E11 fresh AGMs; AGMex: AGM after explant culture; AGMcy: AGM after explant in presence of cyclopamine; CD31p: CD31 positive; CD31n: CD31 negative; KITp: c-Kit positive; KITn: c-Kit negative; BREp: BRE-GFP positive; BREn: BRE-GFP negative

Repositories


GEO

GSE76253

ArrayExpress

E-GEOD-76253

ENA

SRP067686

BioProject

PRJNA306728

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