Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 2
Release date: Sep 4 2012
Last update date: Jul 20 2018
Access: Public
Chemicals: Cholesterol, Glucose
Dataset link Adiponectin regulates expression of hepatic genes critical for glucose and lipid metabolism.

Experimental Protocol


Mice were fasted overnight before euthanization. Liver tissues from WT or adiponectin KO male mice (n = 9-10) at 12 weeks of age were harvested and subjected to total RNA extraction using an RNeasy Plus Mini Kit (Qiagen, Valencia, CA). Total RNA from nine to ten mice of the same strain was pooled together as one biological sample. The mRNA sequencing samples were prepared using the Illumina sample preparation protocol (RS-930-1001, Illumina, Inc. San Diego, CA). The cDNA fragments of 200–250 bp were purified on an agarose gel and then enriched by PCR with Phusion polymerase. The cDNA libraries were sequenced by an Illumina Genome analyzer II at the Whitehead Genome Technology Core.

Repositories


GEO

GSE38144

ArrayExpress

E-GEOD-38144

ENA

SRP013331

BioProject

PRJNA167280

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