Computational protocol: Fluoxetine treatment is effective in a rat model of childhood-induced post-traumatic stress disorder

Similar protocols

Protocol publication

[…] Adapted from Pellow et al. Following 2 min habituation to the testing room rats were placed on the central-platform of the maze facing an open arm (110 × 110 cm, 70 cm above the floor; two opposing open arms/closed arms surrounded by 40 cm high opaque walls on three sides; the maze is covered with a black plexiglas; full light illumination), and were allowed to explore the maze for 5 min. Rats’ behavior was tracked, recorded, and analyzed by the EthoVision XT8 tracking system (Noldus Information Technology, Wageningen, The Netherlands). Behavioral measures included distance covered, duration of stay, and number of entrances in the different arms of the maze. [...] Weight of animals was analyzed using repeated measures two-way analysis of variance (ANOVA) with the stress (control, JVS) and the drug treatment (no FLX, FLXjuv, FLXadlt) as factors. Fluid intake was analyzed separately for the two treatment periods using repeated measures two-way ANOVA with the stress (control, JVS) and the drug treatment (water, FLX) as factors.Behavioral differences in the EPM were first analyzed by using two-way ANOVA with the stress (control, JVS) and the drug treatment (no FLX, FLXjuv, FLXadlt) as factors, followed by Bonferroni post hoc tests for the drug treatment factor when needed. Then, individual behavioral profiling was applied and the distribution of affected vs. unaffected populations was calculated by using Chi-squared test for goodness of fit.All tests were selected after confirming data meet the assumptions of the tests. Data were analyzed using the IBM SPSS Statistics Software version 19 (IBM, Armonk, NY, USA). […]

Pipeline specifications

Software tools EthoVision, SPSS
Applications Miscellaneous, Macroscope & basic digital camera imaging
Organisms Rattus norvegicus, Homo sapiens
Diseases Wounds and Injuries
Chemicals Fluoxetine, Serotonin