Computational protocol: Crystallization and preliminary X-ray diffraction analysis of the periplasmic domain of the Escherichia coli aspartate receptor Tar and its complex with aspartate

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Protocol publication

[…] X-ray diffraction data were collected under cryogenic conditions by flash-cooling with liquid nitrogen after stepwise transfers of all crystals into the crystallization solution supplemented with 10–35%(v/v) glycerol. Diffraction data for apo-Tar1 and Asp-Tar1 were collected using an ADSC Q270 detector on the AR-NE3A beamline and an ADSC Q315 detector on the BL5A beamline at the Photon Factory, Tsukuba, Japan, respectively. Diffraction data for apo-Tar2 and Asp-Tar2 were collected using a MAR 300HE charge-coupled device (CCD) detector on the BL44XU beamline and an ADSC Quantum315 CCD detector on the BL38B1 beamline at SPring-8, Harima, Japan, respectively. Diffraction images were processed with iMosflm and scaled using SCALA in the CCP4 program suite (Battye et al., 2011; Winn et al., 2011). The crystal data statistics are listed in Table 1. […]

Pipeline specifications

Software tools iMosflm, CCP4
Applications Small-angle scattering, Protein structure analysis
Organisms Escherichia coli, Dipturus trachyderma
Chemicals Ammonium Sulfate, Aspartic Acid, Sodium Chloride