Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 41
Release date: Jul 25 2009
Last update date: Mar 21 2012
Access: Public
Chemicals: Galactose, Glucose, Maltose
Dataset link Transcriptional profiling of an evolved polymorphism in E. coli

Experimental Protocol


Four isolates of E. coli evolved under long-term glucose limitation were grown in glucose-limited chemostat culture to steady state. Each isolate was grown separately (i.e in monoculture) in triplicate and three of the four (CV101, CV103, CV115 and CV116) were grown as a consortium in duplicate. Each experiment, or biological replicate, was sampled twice for monoculture experiments and once for consortium experiments. Total RNA from each sample was competitvely hybridized against total RNA from the common ancestor of the isolates grown at the same time in a separate chemostat using the same feed medium. For monoculture experiments, three hybridizations were done for each experiment: two hybridizations comparison for the first sample and a single hybridization for the second (with the exception of sample Jv116-1A which was only hybridized twice due to lack of sufficient RNA). For consortium experiments, two hybridizations were done for each biological replicate.

Repositories


GEO

GSE17276

ArrayExpress

E-GEOD-17276

BioProject

PRJNA123669

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Contact


Frank Rosenzweig

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