Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 4
Release date: Aug 11 2010
Last update date: Mar 21 2012
Access: Public
Chemicals: Carbohydrates, Cellobiose
Dataset link Cellodextrin utilization by Bifidobacterium breve UCC2003

Experimental Protocol


In order to investigate differences in gene expression patterns of B. breve UCC2003 when grown on cellobiose or cellodextrins as compared to growth on glucose, DNA microarray experiments were performed. Total RNA was isolated from B. breve UCC2003 cultures grown on cellobiose, cellodextrins, or glucose (see Materials and Methods). The cultures were harvested at the time points that ensured that B. breve UCC2003 was metabolizing cellobiose or cellodextrins as opposed to the residual glucose present in the cellodextrin preparation. Analysis of the DNA microarray data was obtained from two independent biological replicates.

Repositories


GEO

GSE17604

ArrayExpress

E-GEOD-17604

BioProject

PRJNA118557

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Contact


Karina Pokusaeva