Computational protocol: Controlled branched-chain amino acids auxotrophy in Listeria monocytogenes allows isoleucine to serve as a host signal and virulence effector

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Protocol publication

[…] Lm ilvD promoter was predicted using BPROM []. The leader peptide was predicted using ApE (http://biologylabs.utah.edu/jorgensen/wayned/ape). Terminator and anti-terminator structures were predicted using PASIFIC [], with the kind help of Adi Millman from the Rotem Sorek lab, Weizmann institute. A scheme of the structures was prepared using Mfold []. […]

Pipeline specifications

Software tools BPROM, PASIFIC, Mfold
Applications Genome annotation, Transcription analysis
Organisms Listeria monocytogenes