Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 16
Release date: Aug 3 2007
Last update date: Aug 10 2018
Access: Public
Taxon: Homo sapiens
Dataset link Gene expression profile of conjunctival epithelial cell lines

Experimental Protocol


Affymetrix U133A Genechip Experimental samples: IOBA-NHC cells (5 samples) Chang conjunctival epithelial cells WK derivative (4 samples) Primary conjunctival epithelial cells from explants (3 samples, obtained from cadaveric human explants) Conjunctival tissue from pterygium study where small piece uninvolved conjunctiva harvested (4 patients' RNA pooled to form one sample, total number of samples: 4) RT and hybridisation 16 hr according to Affymetrix protocol Labeling with biotin Washing microfluidics station 450 Analysis with Genespring GX 7.3.1 RMA normalisation following by normalisation to chip level median signal These processed data used for correlation analysis Further gene level normalisation to primary conjunctival epithelial cells samples for the purpose of fold change analysis to compare expression in IOBA or ChWK cells vs primary conjunctival epithelial cells.

Repositories


GEO

GSE8633

ArrayExpress

E-GEOD-8633

BioProject

PRJNA101817

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Contact


Louis Tong