Computational protocol: Two new cestode species of Tetragonocephalum Shipley & Hornell, 1905 (Lecanicephalidea, Tetragonocephalidae) from Himanturarandalli Last, Manjaji-Matsumoto & Moore (Myliobatiformes, Dasyatidae) from the Gulf of Oman

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Protocol publication

[…] A total of 36 specimens of Himantura randalli was collected from northern waters of the Gulf of Oman, 29 individuals in May 2011 and seven in October 2012. They were caught by local fishermen off Jod, Zarabad, Iran (25°26'58"N, 59°30'29"E). Each specimen was given a unique collection number for author reference. All host individuals were photographed and morphometric and morphological characteristics were recorded to facilitate species identification. Species identity was confirmed using , , and .Host specimens were dissected along the mid-ventral line; spiral intestines were removed and opened by a longitudinal incision. Subsequently, spiral intestines were fixed in 10% seawater buffered formalin, shaken vigorously, and held for approximately seven days. The samples were then transferred to the Zoology Laboratory, School of Biology, University of Tehran for detailed examination of parasitic infection.Spiral intestines and intestinal contents were examined under a stereomicroscope. Tapeworms were carefully removed from the spiral intestine and washed in distilled water for about one hour before being preserved in 70% ethanol. Parasite specimens were prepared as whole mounts for light microscopy observation according to .Whole mounts were studied using a Leica DM500 light microscope. Images of Tetragonocephalum specimens were taken using a Leica ICC50 HD color digital camera mounted on the Leica DM500 light microscope (Buffalo Grove, Illinois, United States) and measurements were taken using the image analysis software Leica Application Suite (LAS EZ v.3.0.0) (Leica 2013). Measurements were analyzed in IBM® SPSS® Statistics Package v.22 (IBMCorp. 2013). All measurements of the reproductive organs were taken from mature proglottids. Measurements are given in micrometers (µm) unless otherwise indicated; they are given as the range followed in parentheses by the mean, number of worms examined, and the total number of measurements if more than one measurement was taken per worm. Illustrations were prepared with Adobe® Illustrator® CC (Adobe Incorp. 2013) based on the drafts sketched under a Reichert Biovar microscope with the aid of a drawing tube.Some scoleces were prepared for ultrastructural studies using SEM following the protocol of . The specimens were sputter coated with approximately 10 nm of gold/palladium, and examined with a field emission scanning election microscope (HIT4160102, Hitachi, Tokyo, Japan) at the School of Electrical and Computer Engineering (ECE), University of Tehran. Microthrix terminology follows .School of Electrical and Computer Engineering Type and voucher specimens are deposited at The Zoological Museum, University of Tehran, Tehran, Iran (ZUTC), and The Natural History Museum, London, England (BMNH).Zoological Museum, University of Tehran, Tehran, Iran Natural History Museum, London, England […]

Pipeline specifications

Software tools SPSS, Adobe Illustrator
Application Miscellaneous