Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 40
Release date: Mar 15 2012
Last update date: Nov 9 2017
Access: Public
Diseases: Brain Stem Neoplasms
Dataset link Id proteins synchronize stemness and anchorage to the niche of neural stem cells

Experimental Protocol


We generated Id-cTKO mice carrying a Cre-recombinase-oestrogen-receptor-T2 (Cre-ER) allele targeted to the ubiquitously expressed ROSA26 locus (Id-cTKO-Rosa-Cre-ER). In this system, 4-hydroxytamoxifen (4-OHT) releases the Cre recombinase inhibition and allows recombination of genomic loxP sites. Indeed, efficient deletion of Id1 and Id2 with complete loss of Id protein expression was detectable after treatment of NSCs with 4-OHT for 72 h. Total RNA was extracted from triplicate samples of Id-cTKO-Rosa-Cre-ER NSCs treated for different times (6 h, 12 h, 18 h, 24 h, 48 h, 96 h, 144 h) with 4-OHT or control vehicle and used for analysis on Illumina MouseWG-6 expression BeadChip. The raw array data was normalized using the Bioconductor package Lumi using quantile normalization.

Repositories


GEO

GSE36488

ArrayExpress

E-GEOD-36488

BioProject

PRJNA153527

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Contact


Mario Lauria