Similar protocols

Protocol publication

[…] All images were similarly analyzed with ImageJ. For quantification of fluorogold tracing automatic cell counting was done using custom-written tools. To quantify tDimer2, NeuN and GABA-positive neurons, manual counting was performed, since the high neuronal density in str. pyr. prevented reliable automatic counting. [...] Statistical analyses were performed using SPSS Statistics 21 (IBM, NY, USA) or Matlab. Data were tested for normal distribution by the Shapiro–Wilk test. Normally distributed data were tested for significant differences (*p<0.05, **p<0.01 and ***p<0.001) using paired t-test, unpaired t-test or one-way repeated-measures analysis of variance with Bonferroni-corrected post hoc analysis. Not normally distributed data were tested with the nonparametric Mann–Whitney U-test. The circular statistics toolbox was used to test for significant differences in the phase locking data. Data are presented as mean ±SEM. No statistical measures were used to estimate sample size since effect size was unknown. Investigators were not blinded to the group allocation during the experiments. Unsupervised analysis software was used if possible to preclude investigator biases. Summary of performed statistical analysis is summarized in . […]

Pipeline specifications

Software tools ImageJ, SPSS, CircStat
Applications Miscellaneous, Microscopic phenotype analysis
Organisms Mus musculus