Computational protocol: Treatment of surgical brain injury by immune tolerance induced by intrathymic and hepatic portal vein injection of brain antigens

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Protocol publication

[…] The protocol of above mentioned blood sampling was as follows. All antibodies were purchased from BioLegend. Antibodies were directly marked with one of the following fluorescent tags: fluorescein isothiocyanate (FITC), phycoerythrin (PE), or allophycocyanin (APC). Anti-CD3, anti-CD4, anti-CD8a antibodies and isotypes were used to react with rat antigens. Cell surface phenotype and sorting were recognized using a flow cytometer (BD biosciences), and the data were analyzed using FlowJo 7.6.1 software. [...] Date were presented as means ± SD. Statistical analyses were performed with SPSS software (version IBM SPSS Statistics 22.0) and cartograms were drew with GraphPad Prism software (version 5.01). One-way ANOVA and LSD test were used to determine the significance of differences among subgroups. Independent-Samples t Test was used to compare the differences between Group C1 (SBI+ intrathymic injection of MBP) and Group C2 (SBI+ hepatic portal vein injection of MBP), between Group C2 (SBI+ hepatic portal vein injection of MBP) and Group D2 (SBI+ hepatic portal vein injection of autogeneic brain cell suspension). Two-way ANOVA with Bonferroni post hoc test were performed for multiple comparisons (Namely: thymus and hepatic portal vein served as two factors; normal saline, MBP and autogeneic brain cell suspension served as three levels). P < 0.05 was considered significant. […]

Pipeline specifications

Software tools FlowJo, SPSS
Applications Miscellaneous, Flow cytometry
Diseases Brain Edema, Cerebrovascular Trauma