Dataset features

Specifications


Application: Gene expression microarray analysis
Number of samples: 14
Release date: Dec 12 2016
Last update date: May 11 2017
Access: Public
Dataset link Expression data from meduallar thymic epithelial cells and thymic or splenic B cells

Experimental Protocol


Mature EpCAM+MHC-II high mTECs, Immature EpCAM+MHC-II low mTECs, and EpCAM+Ly51+ cTECs were flow-sorted from thymi isolated from thymi of C57BL/6 6weeks old mice. CD19+Aire.GFP+ thymic B cells and CD19+ Splenic B cells were flow sorted from thyme or spleen, respectively, of C57BL/6 Adig 6weeks old mice. Typically, 3 mice were pooled to obtain 30-60 thousand sorted TECs or thymic B cells, which were then analyzed by gene expression profiling. Specifically, total RNA was extracted from ~30,000 pooled sorted cells using Trizol. Purified total RNA was then amplified using the MessageAmp RNA kit (Ambion). Biotinylated cRNA was then hybridized to Affymetrix Mouse Gene 1-ST arrays by the genomics core at the Weizmann Institute.

Repositories


GEO

GSE89965

BioProject

PRJNA354356

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Contact


Jakub Abramson