|Application:||Gene expression microarray analysis|
|Number of samples:||14|
|Release date:||Dec 12 2016|
|Last update date:||May 11 2017|
|Dataset link||Expression data from meduallar thymic epithelial cells and thymic or splenic B cells|
Mature EpCAM+MHC-II high mTECs, Immature EpCAM+MHC-II low mTECs, and EpCAM+Ly51+ cTECs were flow-sorted from thymi isolated from thymi of C57BL/6 6weeks old mice. CD19+Aire.GFP+ thymic B cells and CD19+ Splenic B cells were flow sorted from thyme or spleen, respectively, of C57BL/6 Adig 6weeks old mice. Typically, 3 mice were pooled to obtain 30-60 thousand sorted TECs or thymic B cells, which were then analyzed by gene expression profiling. Specifically, total RNA was extracted from ~30,000 pooled sorted cells using Trizol. Purified total RNA was then amplified using the MessageAmp RNA kit (Ambion). Biotinylated cRNA was then hybridized to Affymetrix Mouse Gene 1-ST arrays by the genomics core at the Weizmann Institute.