Dataset features

Specifications


Application: RNA-seq analysis
Number of samples: 12
Release date: Jan 30 2016
Last update date: Aug 23 2018
Access: Public
Diseases: Malaria
Dataset link Next generation sequencing analysis of transcriptomes of Plasmodium falciparum male and female gametocytes

Experimental Protocol


A 12 RNA-seq study was performed. Based on GFP intensity, equal number (4-5.5 X 10 6 ) of mature male and female gametocytes were separated by cell-sorter. Total RNA with and without rRNA depletion were processed for generation libraries using the Illumina TruSeq Stranded mRNA Library Preparation kit (Illumina Inc., San Diego, CA). Libraries were sequenced on an Illumina HiSeq 2500 in Rapid Run mode using 100 nucleotide single read sequencing. Altogether, this study investigated three experimental replicates of transcriptomes for mRNA and rRNA from male and female gametocytes (12 samples)

Repositories


GEO

GSE77398

ENA

SRP069075

BioProject

PRJNA310220

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Contact


Jun Miao