|Application:||Gene expression microarray analysis|
|Number of samples:||41|
|Release date:||Dec 16 2016|
|Last update date:||Feb 2 2018|
|Dataset link||Temporal Gene Expression Profiling of Astrocyte Precursors During Development|
To begin dissecting the intermediate stages of astrocyte development, we performed in vivo temporal gene expression profiling of astrocyte precursor cells from the spinal cord of Glast-dsRed mice. Glast is a glutamate transporter and its induction coincides with the gliogenic switch and continues into mature astrocytes in the spinal cord. We chose 6 time points between mE12.5, the initiation of gliogenesis, and mP7, an early post-natal stage where astrocytes express mature markers. During this developmental interval, Glast-expressing cells emerge from the VZ, populating both the mantle zone and the white matter. However, a subset of Glast-expressing cells remains in the ventricular zone (VZ), where progenitor cells are located, marking cell populations in various lineages and differentiative states during spinal cord development. To sub-fractionate prospective Glast-expressing astrocyte precursors from other potential Glast-expressing lineages, we used the cell surface marker LexA/CD15, which has been used previously to purify astrocytes and various progenitor populations. Upon dissection of glast-dsRed spinal cords, we incubated dissociated cells with CD15 antibody prior to FACS isolation. We isolated 3 populations at each time point: Glast-dsRed+/CD15- (henceforth +/-), Glast-dsRed+/CD15+ (henceforth +/+), Glast-dsRed-/CD15- (henceforth -/-). Please note that "-/+" and "+/-" are the same.