Dataset features

Specifications


Application: ChIP-seq analysis
Number of samples: 5
Release date: Mar 27 2014
Last update date: Jul 20 2018
Access: Public
Dataset link Induced Cdx2 binding in embryonic stem cells and endodermal cells

Experimental Protocol


In this study, we characterize the binding of Cdx2 in embryonic stem cells, endodermal cells, and progenitor motor neurons using V5- or FLAG-tagged doxycycline inducible Cdx2 ESC lines (iCdx2). Endoderm and progenitor motor neurons are generated from the ES cells using directed differentiation approaches. The cells are then exposed to Dox to express the tagged Cdx2 construct. The genome-wide binding of the induced full-length Cdx2 transcription factor is profiled using ChIP-seq with an anti-V5 or anti-FLAG antibody. We also examine the binding behavior of a truncated version of the Cdx2 protein, where a protein interaction domain contained in the first 59 amino acids has been deleted. An appropriate pseudo-IP control experiment for these ChIP-seq experiments has been previously submitted under accession number GSM766062.

Repositories


GEO

GSE39435

ArrayExpress

E-GEOD-39435

ENA

SRP014446

BioProject

PRJNA170870

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Contact


Shaun Mahony