Computational protocol: Prevalence and genetic characteristics of Saffold cardiovirus in China from 2009 to 2012

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Protocol publication

[…] The sequences obtained from the study were submitted to NCBI with the GenBank Accession Numbers KJ944637–KJ944718. The genomic sequences were assembled using Lasergene's DNA SeqMan software (version 7.1.0, DNA Star Inc. Madison, WI, USA). The MEGA program (version 6.0, Sudhir Kumar, Arizona State University) was used for alignments and phylogenetic tree construction by maximum likelihood method using 1000 bootstrap pseudo replicates. Nucleotide identities among strains were calculated using BioEdit (version 7.13, www.mbio.ncsu.edu/bioedit/bioedit.html). […]

Pipeline specifications

Software tools MEGA, BioEdit
Application Phylogenetics
Organisms Homo sapiens